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Molecular verification of the intestinal parasites among two breeds of cow from Sagaing Region
https://meral.edu.mm/records/9469
https://meral.edu.mm/records/9469972b579f-f9fa-40ac-bf80-2203fa9d0db6
7e990331-b25e-47fe-9dca-b8bab16990d6
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Journal article | ||||||
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Publication | ||||||
Title | ||||||
Title | Molecular verification of the intestinal parasites among two breeds of cow from Sagaing Region | |||||
Language | en | |||||
Publication date | 2022-12-31 | |||||
Authors | ||||||
Nyein Thi Aung | ||||||
Myo Pa Pa Thet Hnin Htwe Aung | ||||||
Eiji Nagayasu | ||||||
Nyein Nyein Hlaing | ||||||
Description | ||||||
Abstract Livestock farming, particularly rearing of two breeds of cattle (i.e dairy and ploughing cattle) is traditionally practiced by rural people in Myanmar like in other developing countries. Gastrointestinal (GI) parasitic infection is a serious issue in cattle management. The routine diagnosis of GI nematode infections is traditionally based on counting their eggs in a microscopic chamber using a faecal flotation method. This provides little information on the infecting species. Agar plate culture is superior to routine microscopic examination in detection of S. stercoralis and hookworms. Thus combination of methods yielded more different types of intestinal nematodes that could rarely be detected by microscopy alone. Moreover, PCR-based methods developed for the identification and detection of various parasite species. Thus this study was performed on specific identification of various cow intestinal parasites by molecular technique of PCR and sequencing, in addition to the microscopy and culture techniques, among two breeds of cows in Sagaing region. In the present study consisting common nematodes by using sugar floatation method and agar plate culture. Moreover, molecular identification by PCR and subsequent sequencing of 18SrDNA marker revealed nine species of intestinal nematodes including Trichostrongylus colubriformis, Paroiqolaimella micrura, Haemonchus contortus, Zoniolaimus mawsonae, Fictor stercorarius, Rhabditis sp., Necator americanus, Ancylostoma caninum, and Panaqrolaimus davidi among the two breeds of studied cattle. The present study highlighted the essential role of molecular techniques in addition to the conventional diagnostic methods for identification down to the species level of the parasites. |
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Keywords | ||||||
Gastrointestinal parasites, sugar floatation and culture method, molecular identification | ||||||
Journal articles | ||||||
2022 | ||||||
University of Yangon Research Journal 2022 | ||||||
523-532 | ||||||
Vol. 11, No.2 |