{"created":"2024-05-08T04:08:25.344433+00:00","id":9469,"links":{},"metadata":{"_buckets":{"deposit":"7e990331-b25e-47fe-9dca-b8bab16990d6"},"_deposit":{"created_by":235,"id":"9469","owner":"235","owners":[235],"owners_ext":{"displayname":"","email":"thaungbiakpar@gmail.com","username":""},"pid":{"revision_id":0,"type":"depid","value":"9469"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/00009469","sets":["1582963390870:1582967797636"]},"author_link":[],"item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Molecular verification of the intestinal parasites among two breeds of cow from Sagaing Region","subitem_1551255648112":"en"}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"Abstract\nLivestock farming, particularly rearing of two breeds of cattle (i.e dairy and ploughing cattle) is\ntraditionally practiced by rural people in Myanmar like in other developing countries.\nGastrointestinal (GI) parasitic infection is a serious issue in cattle management. The routine\ndiagnosis of GI nematode infections is traditionally based on counting their eggs in a\nmicroscopic chamber using a faecal flotation method. This provides little information on the\ninfecting species. Agar plate culture is superior to routine microscopic examination in detection\nof S. stercoralis and hookworms. Thus combination of methods yielded more different types of\nintestinal nematodes that could rarely be detected by microscopy alone. Moreover, PCR-based\nmethods developed for the identification and detection of various parasite species. Thus this\nstudy was performed on specific identification of various cow intestinal parasites by molecular\ntechnique of PCR and sequencing, in addition to the microscopy and culture techniques, among\ntwo breeds of cows in Sagaing region. In the present study consisting common nematodes by\nusing sugar floatation method and agar plate culture. Moreover, molecular identification by\nPCR and subsequent sequencing of 18SrDNA marker revealed nine species of intestinal\nnematodes including Trichostrongylus colubriformis, Paroiqolaimella micrura, Haemonchus\ncontortus, Zoniolaimus mawsonae, Fictor stercorarius, Rhabditis sp., Necator americanus,\nAncylostoma caninum, and Panaqrolaimus davidi among the two breeds of studied cattle. The\npresent study highlighted the essential role of molecular techniques in addition to the\nconventional diagnostic methods for identification down to the species level of the parasites."}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value_mlt":[{"interim":"Gastrointestinal parasites"},{"interim":"sugar floatation and culture method"},{"interim":"molecular identification"}]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-05-08"}],"displaytype":"preview","filename":"Nyein Thi Aung (523 to 532).pdf","filesize":[{"value":"293 KB"}],"format":"application/pdf","licensetype":"license_0","url":{"url":"https://meral.edu.mm/record/9469/files/Nyein Thi Aung (523 to 532).pdf"},"version_id":"19140b90-fe59-4c0c-b5a0-8f0b5dd30ff7"}]},"item_1583103131163":{"attribute_name":"Journal articles","attribute_value_mlt":[{"subitem_issue":"2022","subitem_journal_title":"University of Yangon Research Journal 2022","subitem_pages":"523-532","subitem_volume":"Vol. 11, No.2"}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Nyein Thi Aung"},{"subitem_authors_fullname":"Myo Pa Pa Thet Hnin Htwe Aung"},{"subitem_authors_fullname":"Eiji Nagayasu"},{"subitem_authors_fullname":"Nyein Nyein Hlaing"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Journal article"}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2022-12-31"},"item_title":"Molecular verification of the intestinal parasites among two breeds of cow from Sagaing Region","item_type_id":"21","owner":"235","path":["1582967797636"],"publish_date":"2024-05-08","publish_status":"0","recid":"9469","relation_version_is_last":true,"title":["Molecular verification of the intestinal parasites among two breeds of cow from Sagaing Region"],"weko_creator_id":"235","weko_shared_id":-1},"updated":"2024-05-08T04:20:10.414952+00:00"}