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  1. Yadanabon University
  2. Department of Chemistry

"Structural Identification and Evaluation of Some Biological Activities of Cyclo (D-Pro-L-Val) from thePseudomonas aeruginosa Schroeter"

https://meral.edu.mm/records/8411
https://meral.edu.mm/records/8411
f058fc8b-122a-47db-a3d0-5fcafb26ca55
7dbbb829-92e8-4ea2-a8f7-1d0701d01b85
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Su Su Swe Su.pdf (694 KB)
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Publication type
Journal article
Upload type
Publication
Title
Title "Structural Identification and Evaluation of Some Biological Activities of Cyclo (D-Pro-L-Val) from thePseudomonas aeruginosa Schroeter"
Language en
Publication date 2022-08-12
Authors
Su Swe Su
Suu Suu Win
Chaw Theingi Khin
Description
"This research deals with the studies of identification and evaluation of some biological
activities of bioactive secondary metabolite compound of Pseudomonas aeruginosa isolated
from the clinical soil sample. The soil sample was collected from Insein General Hospital,
Yangon Region. The isolated P. aeruginosa strain was characterized by microscopic
examination, biochemical tests and confirmed by 16S rRNA sequencing technique. The
chloroform extract was prepared from the P. aeruginosa cultured in a large scale of nutrient
broth medium.Diketopiperazines, such as, cyclo-(D-Pro-L-Val) was isolatedfrom the silica
gel chromatographic separation on the chloroform extract. The isolated secondary
metabolite was structurally identified by using modern NMR spectroscopic techniques such
as 1H NMR(Proton Nuclear Magnetic Resonance) , 13C NMR (Carbon-13 Nuclear Magnetic
Resonance spectroscopy), COSY (¹H-¹H Correlation Spectroscopy), HSQC (Heteronuclear
Single Quantum Coherence), ROESY (Rotating frame Overhause Effect Spectroscopy), HMBC
spectroscopies (Heteronuclear Multiple Bond Correlation), and MS (mass spectrometry).
The isolated compound, cyclo(D-Pro-L-Val) (IC50 = 0.93 μg /mL) was found to exhibit good
in vitro cytotoxic activity against human lung cancercell A-549. In addition, cyclo(D-Pro-LVal)
(IC50 = 64.12 μM) has mild inhibited in anti-inflammatoryactivity."
Keywords
"cyclo(D-Pro-L-Val), modern NMR spectroscopic techniques, in vitro cytotoxicity activity and anti-inflammatoryactivity
Journal articles
2022
Yadanabon University Research Journal
211-219
Vol.12, No.1
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