{"_buckets": {"deposit": "f227e1aa-85d3-43a0-840d-73753a4baa0c"}, "_deposit": {"id": "3136", "owners": [], "pid": {"revision_id": 0, "type": "recid", "value": "3136"}, "status": "published"}, "_oai": {"id": "oai:meral.edu.mm:recid/3136", "sets": ["user-um1"]}, "communities": ["um1"], "item_1583103067471": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes", "subitem_1551255648112": ""}]}, "item_1583103085720": {"attribute_name": "Description", "attribute_value_mlt": [{"interim": "\u003cp\u003eBackground: Skin-derived antimicrobial peptides, such as human b-defensins and cathelicidins, actively\u003cbr\u003e\ncontribute to host defense by inactivating microorganisms. Catestatin, a neuroendocrine peptide that\u003cbr\u003e\naffects human autonomic functions, has recently been detected in keratinocytes upon injury/infection\u003cbr\u003e\nwhere it inhibits the growth of pathogens. Human catestatin exhibits three single nucleotide\u0026nbsp;polymorphisms: Gly364Ser, Pro370Leu, and Arg374Gln.\u003cbr\u003e\nObjective: To investigate the effects of human catestatin and its variants on keratinocyte migration and\u0026nbsp;proliferation, and to elucidate the possible signaling mechanisms involved.\u003cbr\u003e\nMethods: The migration of normal human keratinocytes was analyzed using Boyden microchamber\u0026nbsp;assay and in vitro wound closure assay. Cell proliferation was evaluated by BrdU incorporation, cell count\u0026nbsp;assay and cell cycle analysis. Intracellular Ca2+ mobilization was measured using a fluorescent calcium\u0026nbsp;assay kit. The phosphorylation of epidermal growth factor receptor (EGFR), Akt, and MAPKs was\u0026nbsp;determined by Western blotting.\u003cbr\u003e\nResults: Catestatin and its variants dose-dependently enhanced keratinocyte migration and proliferation.\u0026nbsp;Moreover, catestatin peptides increased intracellular Ca2+ mobilization and induced the\u0026nbsp;phosphorylation of EGFR, Akt, extracellular signal-regulated kinase (ERK), and p38 in keratinocytes.\u0026nbsp;The induction of keratinocyte migration and proliferation by catestatin peptides involved G-proteins,\u0026nbsp;phospholipase C, EGFR, PI3-kinase, ERK, and p38, as evidenced by the specific inhibitory effects of\u0026nbsp;pertussis toxin (G-protein inhibitor), U-73122 (phospholipase C inhibitor), AG1478 (EGFR inhibitor),\u0026nbsp;anti-EGFR antibody, wortmannin (PI3-kinase inhibitor), U0126 (ERK inhibitor), and SB203580 (p38\u0026nbsp;inhibitor), respectively.\u003cbr\u003e\nConclusion: Besides inhibiting the growth of skin pathogens, catestatin peptides may also contribute to\u003cbr\u003e\ncutaneous wound closure by enhancing keratinocyte migration and proliferation at the wound site.\u003c/p\u003e"}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value": []}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2019-10-10"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "Human catestatin enhances migration and proliferation.pdf", "filesize": [{"value": "2933 Kb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_0", "mimetype": "application/pdf", "size": 2933000.0, "url": {"url": "https://meral.edu.mm/record/3136/files/Human catestatin enhances migration and proliferation.pdf"}, "version_id": "8fb1b114-150c-46f6-b59d-0aaf4c8371a2"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{"subitem_issue": "", "subitem_journal_title": "Journal of Dermatological Science", "subitem_pages": "108-118", "subitem_volume": "64"}]}, "item_1583103147082": {"attribute_name": "Conference papers", "attribute_value_mlt": [{"subitem_acronym": "", "subitem_c_date": "", "subitem_conference_title": "", "subitem_part": "", "subitem_place": "", "subitem_session": "", "subitem_website": ""}]}, "item_1583103211336": {"attribute_name": "Books/reports/chapters", "attribute_value_mlt": [{"subitem_book_title": "", "subitem_isbn": "", "subitem_pages": "", "subitem_place": "", "subitem_publisher": ""}]}, "item_1583103233624": {"attribute_name": "Thesis/dissertations", "attribute_value_mlt": [{"subitem_awarding_university": "", "subitem_supervisor(s)": [{"subitem_supervisor": ""}]}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Imranul Hoq"}, {"subitem_authors_fullname": "Francois Niyonsaba"}, {"subitem_authors_fullname": "Hiroko Ushio"}, {"subitem_authors_fullname": "Gyi Aung"}, {"subitem_authors_fullname": "Ko Okumura"}, {"subitem_authors_fullname": "Hideoki Ogawa"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Journal article"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2011-10-19"}, "item_1583159847033": {"attribute_name": "Identifier", "attribute_value": "10.5281/zenodo.3478836"}, "item_title": "Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes", "item_type_id": "21", "owner": "1", "path": ["1596631591423"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000003136", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2019-10-10"}, "publish_date": "2019-10-10", "publish_status": "0", "recid": "3136", "relation": {}, "relation_version_is_last": true, "title": ["Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes"], "weko_shared_id": -1}