{"created":"2020-08-30T20:01:24.656664+00:00","id":3136,"links":{},"metadata":{"_buckets":{"deposit":"f227e1aa-85d3-43a0-840d-73753a4baa0c"},"_deposit":{"id":"3136","owners":[],"pid":{"revision_id":0,"type":"recid","value":"3136"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/3136","sets":["1582963366982:1596631591423"]},"communities":["um1"],"item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes","subitem_1551255648112":""}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"<p>Background: Skin-derived antimicrobial peptides, such as human b-defensins and cathelicidins, actively<br>\ncontribute to host defense by inactivating microorganisms. Catestatin, a neuroendocrine peptide that<br>\naffects human autonomic functions, has recently been detected in keratinocytes upon injury/infection<br>\nwhere it inhibits the growth of pathogens. Human catestatin exhibits three single nucleotide&nbsp;polymorphisms: Gly364Ser, Pro370Leu, and Arg374Gln.<br>\nObjective: To investigate the effects of human catestatin and its variants on keratinocyte migration and&nbsp;proliferation, and to elucidate the possible signaling mechanisms involved.<br>\nMethods: The migration of normal human keratinocytes was analyzed using Boyden microchamber&nbsp;assay and in vitro wound closure assay. Cell proliferation was evaluated by BrdU incorporation, cell count&nbsp;assay and cell cycle analysis. Intracellular Ca2+ mobilization was measured using a fluorescent calcium&nbsp;assay kit. The phosphorylation of epidermal growth factor receptor (EGFR), Akt, and MAPKs was&nbsp;determined by Western blotting.<br>\nResults: Catestatin and its variants dose-dependently enhanced keratinocyte migration and proliferation.&nbsp;Moreover, catestatin peptides increased intracellular Ca2+ mobilization and induced the&nbsp;phosphorylation of EGFR, Akt, extracellular signal-regulated kinase (ERK), and p38 in keratinocytes.&nbsp;The induction of keratinocyte migration and proliferation by catestatin peptides involved G-proteins,&nbsp;phospholipase C, EGFR, PI3-kinase, ERK, and p38, as evidenced by the specific inhibitory effects of&nbsp;pertussis toxin (G-protein inhibitor), U-73122 (phospholipase C inhibitor), AG1478 (EGFR inhibitor),&nbsp;anti-EGFR antibody, wortmannin (PI3-kinase inhibitor), U0126 (ERK inhibitor), and SB203580 (p38&nbsp;inhibitor), respectively.<br>\nConclusion: Besides inhibiting the growth of skin pathogens, catestatin peptides may also contribute to<br>\ncutaneous wound closure by enhancing keratinocyte migration and proliferation at the wound site.</p>"}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value":[]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-10-10"}],"displaytype":"preview","filename":"Human catestatin enhances migration and proliferation.pdf","filesize":[{"value":"2933 Kb"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://meral.edu.mm/record/3136/files/Human catestatin enhances migration and proliferation.pdf"},"version_id":"8fb1b114-150c-46f6-b59d-0aaf4c8371a2"}]},"item_1583103131163":{"attribute_name":"Journal articles","attribute_value_mlt":[{"subitem_issue":"","subitem_journal_title":"Journal of Dermatological Science","subitem_pages":"108-118","subitem_volume":"64"}]},"item_1583103147082":{"attribute_name":"Conference papers","attribute_value_mlt":[{"subitem_acronym":"","subitem_c_date":"","subitem_conference_title":"","subitem_part":"","subitem_place":"","subitem_session":"","subitem_website":""}]},"item_1583103211336":{"attribute_name":"Books/reports/chapters","attribute_value_mlt":[{"subitem_book_title":"","subitem_isbn":"","subitem_pages":"","subitem_place":"","subitem_publisher":""}]},"item_1583103233624":{"attribute_name":"Thesis/dissertations","attribute_value_mlt":[{"subitem_awarding_university":"","subitem_supervisor(s)":[{"subitem_supervisor":""}]}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Imranul Hoq"},{"subitem_authors_fullname":"Francois Niyonsaba"},{"subitem_authors_fullname":"Hiroko Ushio"},{"subitem_authors_fullname":"Gyi Aung"},{"subitem_authors_fullname":"Ko Okumura"},{"subitem_authors_fullname":"Hideoki Ogawa"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Journal article"}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2011-10-19"},"item_1583159847033":{"attribute_name":"Identifier","attribute_value":"10.5281/zenodo.3478836"},"item_title":"Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes","item_type_id":"21","owner":"1","path":["1596631591423"],"publish_date":"2019-10-10","publish_status":"0","recid":"3136","relation_version_is_last":true,"title":["Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2021-12-13T05:48:40.597416+00:00"}