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  1. Yezin Agricultural University
  2. Department of Agricultural Biotechnology

Development of cDNA Derived SSR Markers and Their Efficiency in Diversity Assessment of Cymbidium Accessions

http://hdl.handle.net/20.500.12678/0000000219
a599e863-af93-47f2-a5b6-d274f9f6a6fe
a9c24871-d41b-4ee5-bc2b-5e4a0c7ae79a
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cDNA cDNA derived SSR markers (Moe et al 2012).jpg (361 Kb)
Publication type Journal article
Upload Type Publication
Title
Development of cDNA Derived SSR Markers and Their Efficiency in Diversity Assessment of Cymbidium Accessions
en
Publication date 2012-03
Authors
Moe Kyaw Thu
Woo-Ju Hong
Soon-Wook Kwon
Yong-Jin Park
Description
Cymbidium species are popular flowering plants. Assessment of genetic diversity in cultivated Cymbidium facilitates conservation of germplasm and subsequent cultivar improvement. Thus, it is popular to develop more efficient polymorphic DNA markers. Although more motifs (403) were identified and more primers (206) were designed in the genomic library compare to cDNA library, a large number of successful primers were obtained from the cDNA library (59.9%) than from genomic library (51.1%). However, higher PIC and gene diversity were identified in genomic SSR. The average allele number per locus was also higher in genomic SSRs (7.3) than EST-SSRs
(5.2), among the 24 evaluated Cymbidium accessions. AT/TA was comparatively high in EST-SSRs,
while this motif was not as common in genomic SSRs. The CTT/AAG/TCT/AGA/TTC/GAA and
TGC/GCA/GCT/AGC/CTG/CAG motifs were the most abundant tri-nucleotide sequences in ESTSSRs, while GTT/AAC/TGT/ACA/TTG/CAA was the most frequent in genomic SSRs. The number of
repeats ranged from 3 to 12 in EST-SSRs. Currently, 52 novel polymorphic SSR markers have been
evaluated, which will be useful for germplasm assessments, core set construction, evaluation of
genetic diversity, and marker assisted selection (MAS) based Cymbidium breeding.
Keywords
cDNA library
Journal articles
Electronic Journal of Biotechnology
Conference papaers
Books/reports/chapters
Thesis/dissertations
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