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  1. University of Yangon
  2. Department of Chemistry

Production of Chitosanolytic Bacteria from Soil Sample

https://meral.edu.mm/records/9296
https://meral.edu.mm/records/9296
623e7c3c-ecef-4cf4-926e-d3d98e896e22
8251ffc1-106a-4bc1-9089-6f2e89b45ca4
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Aye Aye Aye Myat (243 to 250 ).pdf (654 KB)
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Publication type
Journal article
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Publication
Title
Title Production of Chitosanolytic Bacteria from Soil Sample
Language en
Publication date 2022-12-31
Authors
Aye Aye Myat
Daw Hla Ngwe
Description
Bacillus megaterium produces penicillin amides used to make synthetic penicillin and several enzymes. This study focuses on showing the presence of Bacillus megaterium in the market area. In this research, the soil samples (A1, A2, A3, A4, A5, A6, A7, A8, A9, and A10) were collected from the Htauk-kyant market area, Yangon Region, and a preliminary screening of the bacteria present in the sample was carried out. The bacteria were isolated from soil samples by the serial dilution plate method and then cultured in nutrient agar medium. Ten bacterial strains (A1 to A10) were isolated and characterized by microscopic examination and gram staining methods. Among these bacterial strains, eight gram-positive bacterial strains: A1, A2, A3, A4, A6, A8, A9, and A10 were identified by phenotypic analysis. The confirmation of chitosanase producing bacteria was performed by qualitative methods such as a chitosan detection agar assay and the DNS (3, 5-dinitrosalicylic acid) assay. In these tests, A2 was found to show chitosanolytic activity.
Keywords
Bacillus megaterium, serial dilution plate method, gram staining test, phenotypic, chitosanolytic activity
Journal articles
2022
University of Yangon Research Journal
243-250
11, No. 1
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