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Item
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ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM THE ROOT NODULES OF VIGNA MUNGO L. (BLACK GRAM)
https://meral.edu.mm/records/9288
https://meral.edu.mm/records/9288eb6a9a44-41d3-496f-a96a-f3a22ed42e01
6dd27a82-c943-4802-881c-735a3a574e3a
Name / File | License | Actions |
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![]() |
Publication type | ||||||
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Journal article | ||||||
Upload type | ||||||
Publication | ||||||
Title | ||||||
Title | ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM THE ROOT NODULES OF VIGNA MUNGO L. (BLACK GRAM) | |||||
Language | en | |||||
Publication date | 2022-12-31 | |||||
Authors | ||||||
Phoo Wint Yee Thaw | ||||||
Description | ||||||
The present study was conducted to isolate and characterize the rhizobia from the root nodules of Vigna mungo L. (Black gram). Plant sample and rood nodules sample were collected from the Hinthada Township, Ayeyarwaddy Region. The experiment was performed at the Microbiology Laboratory, Department of Botany, University of Yangon in 2022. The two bacterial isolates (PBR1 and PBR2) were identified as Rhizobium based on the results of authentication tests (Congo red test, Keto-lactose test, Bromothymol blue test, Glucose- peptone agar test and Hoffer’s alkaline test). On morphological properties, Rhizobium colonies on yeast extract mannitol agar (YEMA) medium were circular, mucoid, glistering, and white with convex elevation and smooth surface. Under microscopic examination, both isolates appeared as Gram- negative, and rod shaped. Biochemical characterization of two Rhizobium isolates showed positive reactions with catalase, methyl red (MR), nitrate reduction and urease while both strains indicated negative reaction with citrate utilization, gelatinase enzyme, indole, Triple Sugar Iron Agar and Vogas Proskauer (VP) test. | ||||||
Keywords | ||||||
Rhizobium, Root nodules, Black gram | ||||||
Journal articles | ||||||
2022 | ||||||
University of Yangon Research Journal | ||||||
163-172 | ||||||
11, No. 1 |