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Item

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ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM THE ROOT NODULES OF VIGNA MUNGO L. (BLACK GRAM)

https://meral.edu.mm/records/9288
eb6a9a44-41d3-496f-a96a-f3a22ed42e01
6dd27a82-c943-4802-881c-735a3a574e3a
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Phoo Phoo Wint Yee Thaw (163 to 172 ).pdf (497 KB)
Publication type
Journal article
Upload type
Publication
Title
Title ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM THE ROOT NODULES OF VIGNA MUNGO L. (BLACK GRAM)
Language en
Publication date 2022-12-31
Authors
Phoo Wint Yee Thaw
Description
The present study was conducted to isolate and characterize the rhizobia from the root nodules of Vigna mungo L. (Black gram). Plant sample and rood nodules sample were collected from the Hinthada Township, Ayeyarwaddy Region. The experiment was performed at the Microbiology Laboratory, Department of Botany, University of Yangon in 2022. The two bacterial isolates (PBR1 and PBR2) were identified as Rhizobium based on the results of authentication tests (Congo red test, Keto-lactose test, Bromothymol blue test, Glucose- peptone agar test and Hoffer’s alkaline test). On morphological properties, Rhizobium colonies on yeast extract mannitol agar (YEMA) medium were circular, mucoid, glistering, and white with convex elevation and smooth surface. Under microscopic examination, both isolates appeared as Gram- negative, and rod shaped. Biochemical characterization of two Rhizobium isolates showed positive reactions with catalase, methyl red (MR), nitrate reduction and urease while both strains indicated negative reaction with citrate utilization, gelatinase enzyme, indole, Triple Sugar Iron Agar and Vogas Proskauer (VP) test.
Keywords
Rhizobium, Root nodules, Black gram
Journal articles
2022
University of Yangon Research Journal
163-172
11, No. 1
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0
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