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  1. Yezin Agricultural University
  2. Department of Plant Pathology

Characterization of Ralstonia solanacearum isolates in Myanmar

http://hdl.handle.net/20.500.12678/0000000090
http://hdl.handle.net/20.500.12678/0000000090
3fbf1814-c4b8-4a8a-a24a-5305984167c9
375c4f04-4019-4dfe-95a7-16f1afb2c89e
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6. 6. Characterization of R. solanacearum in Myanmar.pdf (143 Kb)
Publication type
Journal article
Upload type
Publication
Title
Title Characterization of Ralstonia solanacearum isolates in Myanmar
Language en
Publication date 2014-12-23
Authors
Htct Wai Wai Kyaw
Seint San Aye
Naruto Furuya
Kenichi Tsuchiya
Description
Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases in economically important crops in the world. It is also important disease of solanaceous crops in Myanmar. The objective of this study was to identify the pathogenic isolates from d ifferent hosts and to characterize their race, biovar and phylotype levels. The disease collection was carried out in Shan State which is highland region (Kalaw, Heho), Mandalay Region (PyinOolwin and NyaungOo) and Naypyidaw Region (Yezin) which are relatively lowland areas during October 2012 and April 2013. Isolation was made on selective med ia (Tetrazolium chloride med ia) and identified as R. solanacearum based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Total 32 isolates (14 potato isolates from Shan State, six pepper isolates, six tomato isolates and two eggplant isolates from Naypyidaw Region and four tomato isolates from Mandalay Region) were examined and all isolates tested were amplified R. solanacearum specific band (281 bp) by PCR. All tomato, eggplant and pepper strains belonged to race I biovar 3. Potato strains isolated from Heho area were race 3 biovar 2 and race I biovar 3. At Heho area, 33% of the tested strains belonged to race 3 biovar 2. Furthennore, multi plex PCR was conducted for the discrimination of phylotypes. Race I biovar 3 strains and race 3 biovar 2 strains belonged to phylotype I and phylotype II, respectively. This is the first report of identification and characterization of Myanmar R. solanacearum strains to race, biovar and phylotype.
Keywords
bacterial wilt
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/88
Journal articles
Proceedings of the Eighth Agricultural Research Conference
Conference papaers
Books/reports/chapters
Thesis/dissertations
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