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  1. Yezin Agricultural University
  2. Department of Agricultural Biotechnology

Development of SSR markers to study diversity in the genus Cymbidium

Permalink : http://hdl.handle.net/20.500.12678/0000000069
53c89794-cd1c-4e43-95cd-bb1e18f8e1b2
53f251ea-19b4-4121-86aa-a43a895f53ed
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Cymbidium Cymbidium SSR Marker development.docx (818.5 kB)
sha256: 757988c9e2323e76ee41c32e931d01d63e9836382de55332fbfd2df6557247fa
Publication type Journal article
Upload Type Publication
Title
Development of SSR markers to study diversity in the genus Cymbidium
en
Publication date 2010-07
Authors
Moe Kyaw Thu
Weiguo Zhao
Hong-Seon Song
You-Hyen Kim
Jong-Wook Chung
Young-Il Cho
Pue Hee Park
Ha-Seung Park
Soo-Cheon Chae
Yong-Jin Park
Description
Cymbidium spp. are important potted flowers with extremely high ornamental and economic value. The present study reports the development of 14 new simple sequence repeat (SSR) markers through the construction of an enriched Cymbidium goeringii library and cross-amplification in Cymbidium sinensis and Cymbidium hybridium. Of 525, 322 (61.33%) clones had SSR motifs and among motifs di-nucleotides were predominant and followed by tri-nucleotide and tetra-nucleotide type. In polymorphic analysis using 14 newly developed SSRs, a total of 201 alleles across 96 Cymbidium accessions were detected with an average of 14.4 per locus. The average heterozygosity was 0.394. The average gene diversity and polymorphism information content values were 0.394 and 0.639, respectively. The mean genetic similarity coefficient was 0.4297, indicating a wide genetic variation among the Cymbidium accessions. These newly developed SSRs will be useful tools for genotype identification, germplasm conservation, molecular breeding, and assessments of genetic diversity and population structure in Cymbidium.
Keywords
Cymbidium
Files 818 Kb
Available
2020-05-05
application/vnd.openxmlformats-officedocument.wordprocessingml.document
preview
application/pdf
open_access
Cymbidium SSR Marker development.docx

Journal articles
Biochemical Systematics and Ecology
38
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