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Development of SSR markers to study diversity in the genus Cymbidium
http://hdl.handle.net/20.500.12678/0000000069
http://hdl.handle.net/20.500.12678/000000006953c89794-cd1c-4e43-95cd-bb1e18f8e1b2
53f251ea-19b4-4121-86aa-a43a895f53ed
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Cymbidium SSR Marker development.docx (818 Kb)
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Publication type | ||||||
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Journal article | ||||||
Upload type | ||||||
Publication | ||||||
Title | ||||||
Title | Development of SSR markers to study diversity in the genus Cymbidium | |||||
Language | en | |||||
Publication date | 2010-07 | |||||
Authors | ||||||
Moe Kyaw Thu | ||||||
Weiguo Zhao | ||||||
Hong-Seon Song | ||||||
You-Hyen Kim | ||||||
Jong-Wook Chung | ||||||
Young-Il Cho | ||||||
Pue Hee Park | ||||||
Ha-Seung Park | ||||||
Soo-Cheon Chae | ||||||
Yong-Jin Park | ||||||
Description | ||||||
Cymbidium spp. are important potted flowers with extremely high ornamental and economic value. The present study reports the development of 14 new simple sequence repeat (SSR) markers through the construction of an enriched Cymbidium goeringii library and cross-amplification in Cymbidium sinensis and Cymbidium hybridium. Of 525, 322 (61.33%) clones had SSR motifs and among motifs di-nucleotides were predominant and followed by tri-nucleotide and tetra-nucleotide type. In polymorphic analysis using 14 newly developed SSRs, a total of 201 alleles across 96 Cymbidium accessions were detected with an average of 14.4 per locus. The average heterozygosity was 0.394. The average gene diversity and polymorphism information content values were 0.394 and 0.639, respectively. The mean genetic similarity coefficient was 0.4297, indicating a wide genetic variation among the Cymbidium accessions. These newly developed SSRs will be useful tools for genotype identification, germplasm conservation, molecular breeding, and assessments of genetic diversity and population structure in Cymbidium. |
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Keywords | ||||||
Cymbidium | ||||||
Identifier | https://yauor-yau.archive.knowledgearc.net/handle/123456789/68 | |||||
Journal articles | ||||||
Biochemical Systematics and Ecology | ||||||
38 | ||||||
Conference papaers | ||||||
Books/reports/chapters | ||||||
Thesis/dissertations |