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  1. Yezin Agricultural University
  2. Department of Agricultural Biotechnology

Development of SSR markers to study diversity in the genus Cymbidium

http://hdl.handle.net/20.500.12678/0000000069
http://hdl.handle.net/20.500.12678/0000000069
53c89794-cd1c-4e43-95cd-bb1e18f8e1b2
53f251ea-19b4-4121-86aa-a43a895f53ed
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Cymbidium Cymbidium SSR Marker development.docx (818 Kb)
Publication type
Journal article
Upload type
Publication
Title
Title Development of SSR markers to study diversity in the genus Cymbidium
Language en
Publication date 2010-07
Authors
Moe Kyaw Thu
Weiguo Zhao
Hong-Seon Song
You-Hyen Kim
Jong-Wook Chung
Young-Il Cho
Pue Hee Park
Ha-Seung Park
Soo-Cheon Chae
Yong-Jin Park
Description
Cymbidium spp. are important potted flowers with extremely high ornamental and
economic value. The present study reports the development of 14 new simple sequence
repeat (SSR) markers through the construction of an enriched Cymbidium goeringii library
and cross-amplification in Cymbidium sinensis and Cymbidium hybridium. Of 525, 322
(61.33%) clones had SSR motifs and among motifs di-nucleotides were predominant and
followed by tri-nucleotide and tetra-nucleotide type. In polymorphic analysis using 14
newly developed SSRs, a total of 201 alleles across 96 Cymbidium accessions were detected
with an average of 14.4 per locus. The average heterozygosity was 0.394. The average gene
diversity and polymorphism information content values were 0.394 and 0.639, respectively.
The mean genetic similarity coefficient was 0.4297, indicating a wide genetic variation
among the Cymbidium accessions. These newly developed SSRs will be useful tools
for genotype identification, germplasm conservation, molecular breeding, and assessments
of genetic diversity and population structure in Cymbidium.
Keywords
Cymbidium
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/68
Journal articles
Biochemical Systematics and Ecology
38
Conference papaers
Books/reports/chapters
Thesis/dissertations
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