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The present study was carried out to evaluate callus induction ability of\r selected rice genotypes on media supplemented with two different carbon sources and\r to investigate suitable BAP concentrations on plant regeneration of anther derived\r callus. The selected 19 rice genotypes (14 indica and 5 tropical japonica) were used in\r the experiment. Anthers of each genotype were cultured on N6 medium supplemented\r with two types of carbon source (4% maltose or 4% sucrose), 2mg.L-1 2,4-\r Dichlorophenoxyacetic acid (2,4-D) and 0.5 mg.L1 kinetin for callus induction.\r Anther derived callus was transferred to Murashige and Skoog (MS) medium\r supplemented with (0, 0.5, 1) mg.L-1 6-Benzylaminopurine (BAP), 1mg.L-11-\r Naphthaleneacetic acid (NAA) and 1mg.L-1 l kinetin for regeneration.\r Seventeen out of 19 rice genotypes produced callus on media supplemented\r with maltose while only 13 genotypes produced callus on media supplemented with\r sucrose due to genotype dependency. Tropical japonica was more responsive to\r induce callus from anther than indica among the tested genotypes. Callus induction\r varied from 0.44% to 19.22%. Tropical japonica (Paw San Taung Pyan Hmwe) has\r highest callus induction (19.22%) than the other tested genotypes. Yebaw Sein has\r highest callus induction (6.78%) among the tested indica rice genotypes. Plant\r regeneration from callus varied from 0% to 25.15%. In tropical japonica genotypes,\r Bay Kyar Taung Pyan was recorded maximum green plants regeneration (25.15%),\r while Hnan Kar was (13.33%) among indica genotypes. In this experiment, both MS\r media supplemented with 0.5mg.L-1 BAP and 1mg.L-1 BAP showed maximum green\r plants regeneration although maximum green spots formation occurred only on\r 1mg.L-1 BAP. Maximum green plant was found in Bay Kyar Taung Pyan in tropical\r japonica and Lat Yone Kyi in indica rice."}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value_mlt": [{"interim": "anther culture"}]}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2020-05-05"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "khin soe win MS Thesis 2017.pdf", "filesize": [{"value": "809 Kb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 809000.0, "url": {"url": "https://meral.edu.mm/record/55/files/khin soe win MS Thesis 2017.pdf"}, "version_id": "50a09e8c-469f-4540-b656-01c0290edfd4"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{}]}, "item_1583103147082": {"attribute_name": "Conference papaers", "attribute_value_mlt": [{}]}, "item_1583103211336": {"attribute_name": "Books/reports/chapters", "attribute_value_mlt": [{}]}, "item_1583103233624": {"attribute_name": "Thesis/dissertations", "attribute_value_mlt": [{"subitem_supervisor(s)": []}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Khin Soe Win"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Thesis"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2017-07"}, "item_1583159847033": {"attribute_name": "Identifier", "attribute_value": "https://yauor-yau.archive.knowledgearc.net/handle/123456789/266"}, "item_title": "In Vitro Regeneration of Selected Rice (Oryza sativa L.) Genotypes through Anther Culture", "item_type_id": "21", "owner": "1", "path": ["1582969222431"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000000055", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2020-03-05"}, "publish_date": "2020-03-05", "publish_status": "0", "recid": "55", "relation": {}, "relation_version_is_last": true, "title": ["In Vitro Regeneration of Selected Rice (Oryza sativa L.) Genotypes through Anther Culture"], "weko_shared_id": -1}
  1. Yezin Agricultural University
  2. Department of Horticulture

In Vitro Regeneration of Selected Rice (Oryza sativa L.) Genotypes through Anther Culture

http://hdl.handle.net/20.500.12678/0000000055
http://hdl.handle.net/20.500.12678/0000000055
1cb4e8bf-8127-48ef-8591-6721bef08a94
7dd1995b-7c49-43c4-9a84-e8b6772b2928
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khin khin soe win MS Thesis 2017.pdf (809 Kb)
Publication type
Thesis
Upload type
Publication
Title
Title In Vitro Regeneration of Selected Rice (Oryza sativa L.) Genotypes through Anther Culture
Language en
Publication date 2017-07
Authors
Khin Soe Win
Description
Rice is one of the most important cereal crops in the world. Anther culture,
one of double haploid techniques, is simplest and most efficient technique for rice
breeding. The present study was carried out to evaluate callus induction ability of
selected rice genotypes on media supplemented with two different carbon sources and
to investigate suitable BAP concentrations on plant regeneration of anther derived
callus. The selected 19 rice genotypes (14 indica and 5 tropical japonica) were used in
the experiment. Anthers of each genotype were cultured on N6 medium supplemented
with two types of carbon source (4% maltose or 4% sucrose), 2mg.L-1 2,4-
Dichlorophenoxyacetic acid (2,4-D) and 0.5 mg.L1 kinetin for callus induction.
Anther derived callus was transferred to Murashige and Skoog (MS) medium
supplemented with (0, 0.5, 1) mg.L-1 6-Benzylaminopurine (BAP), 1mg.L-11-
Naphthaleneacetic acid (NAA) and 1mg.L-1 l kinetin for regeneration.
Seventeen out of 19 rice genotypes produced callus on media supplemented
with maltose while only 13 genotypes produced callus on media supplemented with
sucrose due to genotype dependency. Tropical japonica was more responsive to
induce callus from anther than indica among the tested genotypes. Callus induction
varied from 0.44% to 19.22%. Tropical japonica (Paw San Taung Pyan Hmwe) has
highest callus induction (19.22%) than the other tested genotypes. Yebaw Sein has
highest callus induction (6.78%) among the tested indica rice genotypes. Plant
regeneration from callus varied from 0% to 25.15%. In tropical japonica genotypes,
Bay Kyar Taung Pyan was recorded maximum green plants regeneration (25.15%),
while Hnan Kar was (13.33%) among indica genotypes. In this experiment, both MS
media supplemented with 0.5mg.L-1 BAP and 1mg.L-1 BAP showed maximum green
plants regeneration although maximum green spots formation occurred only on
1mg.L-1 BAP. Maximum green plant was found in Bay Kyar Taung Pyan in tropical
japonica and Lat Yone Kyi in indica rice.
Keywords
anther culture
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/266
Journal articles
Conference papaers
Books/reports/chapters
Thesis/dissertations
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