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  1. Yangon Technological University
  2. Department of Chemical Engineering

Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential

http://hdl.handle.net/20.500.12678/0000005325
533f15d6-8048-45f3-a338-ce15a3b9daa6
04d2390e-137c-492d-aadf-2bd02ed0e416
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Adsorption Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potentialEi.pdf (1.5 Mb)
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Publication type Journal article
Upload type Publication
Title
Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential
en
Publication date 2016-07-01
Authors
Ei Ei Htwe
Yuhi Nakama
Hiroshi Tanaka
Hiroyuki Imanaka
Naoyuki Ishida
Koreyoshi Imamura
Description
tThe impact of external electric potential on the adsorption of a protein to base metal surfaces was exam-ined. Hen egg white lysozyme (LSZ) and six types of base metal plates (stainless steel SUS316L (St), Ti, Ta,Zr, Cr, or Ni) were used as the protein and adsorption surface, respectively. LSZ was allowed to adsorb onthe surface under different conditions (surface potential, pH, electrolyte type and concentration, surfacematerial), which was monitored using an ellipsometer. LSZ adsorption was minimized in the potentialrange above a certain threshold and, in the surface potential range below the threshold, decreasing thesurface potential increased the amount of protein adsorbed. The threshold potential for LSZ adsorptionwas shifted toward a positive value with increasing pH and was lower for Ta and Zr than for the others. Adivalent anion salt (K2SO4) as an electrolyte exhibited the adsorption of LSZ in the positive potential rangewhile a monovalent salt (KCl) did not. A comprehensive consideration of the obtained results suggeststhat two modes of interactions, namely the electric force by an external electric field and electrostaticinteractions with ionized surface hydroxyl groups, act on the LSZ molecules and determine the extentof suppression of LSZ adsorption. All these findings appear to support the view that a base metal surfacecan be controlled for the affinity to a protein by manipulating the surface electric potential as has beenreported on some electrode materials.
Keywords
Adsorption
Keywords
Lysozyme
Keywords
Metal oxide
Keywords
External electric potential
Keywords
Ellipsometer
Keywords
Surface hydroxyl group
Keywords
Electrostatic interactiona
Journal articles
Colloids and Surfaces B: Biointerfaces, ELSEVIER (Okayama University) Japan
pp 9_16
147
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