MERAL Myanmar Education Research and Learning Portal
Item
{"_buckets": {"deposit": "04d2390e-137c-492d-aadf-2bd02ed0e416"}, "_deposit": {"created_by": 30, "id": "5325", "owner": "30", "owners": [30], "owners_ext": {"displayname": "", "username": ""}, "pid": {"revision_id": 0, "type": "recid", "value": "5325"}, "status": "published"}, "_oai": {"id": "oai:meral.edu.mm:recid/5325", "sets": ["1596105561292", "user-ytu"]}, "communities": ["ytu"], "item_1583103067471": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential", "subitem_1551255648112": "en"}]}, "item_1583103085720": {"attribute_name": "Description", "attribute_value_mlt": [{"interim": "tThe impact of external electric potential on the adsorption of a protein to base metal surfaces was exam-ined. Hen egg white lysozyme (LSZ) and six types of base metal plates (stainless steel SUS316L (St), Ti, Ta,Zr, Cr, or Ni) were used as the protein and adsorption surface, respectively. LSZ was allowed to adsorb onthe surface under different conditions (surface potential, pH, electrolyte type and concentration, surfacematerial), which was monitored using an ellipsometer. LSZ adsorption was minimized in the potentialrange above a certain threshold and, in the surface potential range below the threshold, decreasing thesurface potential increased the amount of protein adsorbed. The threshold potential for LSZ adsorptionwas shifted toward a positive value with increasing pH and was lower for Ta and Zr than for the others. Adivalent anion salt (K2SO4) as an electrolyte exhibited the adsorption of LSZ in the positive potential rangewhile a monovalent salt (KCl) did not. A comprehensive consideration of the obtained results suggeststhat two modes of interactions, namely the electric force by an external electric field and electrostaticinteractions with ionized surface hydroxyl groups, act on the LSZ molecules and determine the extentof suppression of LSZ adsorption. All these findings appear to support the view that a base metal surfacecan be controlled for the affinity to a protein by manipulating the surface electric potential as has beenreported on some electrode materials."}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value_mlt": [{"interim": "Adsorption"}, {"interim": "Lysozyme"}, {"interim": "Metal oxide"}, {"interim": "External electric potential"}, {"interim": "Ellipsometer"}, {"interim": "Surface hydroxyl group"}, {"interim": "Electrostatic interactiona"}]}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2020-09-10"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potentialEi.pdf", "filesize": [{"value": "1.5 Mb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_0", "mimetype": "application/pdf", "size": 1500000.0, "url": {"url": "https://meral.edu.mm/record/5325/files/Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potentialEi.pdf"}, "version_id": "6f7939c1-4583-4b82-8ad3-a0c2c5399fb3"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{"subitem_journal_title": "Colloids and Surfaces B: Biointerfaces, ELSEVIER (Okayama University) Japan", "subitem_pages": "pp 9_16", "subitem_volume": "147"}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Ei Ei Htwe"}, {"subitem_authors_fullname": "Yuhi Nakama"}, {"subitem_authors_fullname": "Hiroshi Tanaka"}, {"subitem_authors_fullname": "Hiroyuki Imanaka"}, {"subitem_authors_fullname": "Naoyuki Ishida"}, {"subitem_authors_fullname": "Koreyoshi Imamura"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Journal article"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2016-07-01"}, "item_title": "Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential", "item_type_id": "21", "owner": "30", "path": ["1596105561292"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000005325", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2020-09-10"}, "publish_date": "2020-09-10", "publish_status": "0", "recid": "5325", "relation": {}, "relation_version_is_last": true, "title": ["Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential"], "weko_shared_id": -1}
Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential
http://hdl.handle.net/20.500.12678/0000005325
http://hdl.handle.net/20.500.12678/0000005325533f15d6-8048-45f3-a338-ce15a3b9daa6
04d2390e-137c-492d-aadf-2bd02ed0e416
Name / File | License | Actions |
---|---|---|
Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potentialEi.pdf (1.5 Mb)
|
Publication type | ||||||
---|---|---|---|---|---|---|
Journal article | ||||||
Upload type | ||||||
Publication | ||||||
Title | ||||||
Title | Adsorption of lysozyme on base metal surfaces in the presence of anexternal electric potential | |||||
Language | en | |||||
Publication date | 2016-07-01 | |||||
Authors | ||||||
Ei Ei Htwe | ||||||
Yuhi Nakama | ||||||
Hiroshi Tanaka | ||||||
Hiroyuki Imanaka | ||||||
Naoyuki Ishida | ||||||
Koreyoshi Imamura | ||||||
Description | ||||||
tThe impact of external electric potential on the adsorption of a protein to base metal surfaces was exam-ined. Hen egg white lysozyme (LSZ) and six types of base metal plates (stainless steel SUS316L (St), Ti, Ta,Zr, Cr, or Ni) were used as the protein and adsorption surface, respectively. LSZ was allowed to adsorb onthe surface under different conditions (surface potential, pH, electrolyte type and concentration, surfacematerial), which was monitored using an ellipsometer. LSZ adsorption was minimized in the potentialrange above a certain threshold and, in the surface potential range below the threshold, decreasing thesurface potential increased the amount of protein adsorbed. The threshold potential for LSZ adsorptionwas shifted toward a positive value with increasing pH and was lower for Ta and Zr than for the others. Adivalent anion salt (K2SO4) as an electrolyte exhibited the adsorption of LSZ in the positive potential rangewhile a monovalent salt (KCl) did not. A comprehensive consideration of the obtained results suggeststhat two modes of interactions, namely the electric force by an external electric field and electrostaticinteractions with ionized surface hydroxyl groups, act on the LSZ molecules and determine the extentof suppression of LSZ adsorption. All these findings appear to support the view that a base metal surfacecan be controlled for the affinity to a protein by manipulating the surface electric potential as has beenreported on some electrode materials. | ||||||
Keywords | ||||||
Adsorption, Lysozyme, Metal oxide, External electric potential, Ellipsometer, Surface hydroxyl group, Electrostatic interactiona | ||||||
Journal articles | ||||||
Colloids and Surfaces B: Biointerfaces, ELSEVIER (Okayama University) Japan | ||||||
pp 9_16 | ||||||
147 |