{"_buckets": {"deposit": "3a6f1837-0e78-4399-a211-8acbce2cf134"}, "_deposit": {"id": "49", "owners": [], "pid": {"revision_id": 0, "type": "recid", "value": "49"}, "status": "published"}, "_oai": {"id": "oai:meral.edu.mm:recid/49", "sets": ["1582969313627", "user-yau"]}, "communities": ["yau"], "control_number": "49", "item_1583103067471": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Distribution and Frequencie of SSR Motifs in the Chrysanthemum SSR-enrich Library through 454 Pyrosequencing Technology", "subitem_1551255648112": "en"}]}, "item_1583103085720": {"attribute_name": "Description", "attribute_value_mlt": [{"interim": "Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum,\r which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted\r DNA from Dendranthema grandiflorum (‘Smileball’) to construct a simple sequence repeat (SSR)-\r enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer\r FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the\r 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp.\r Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375,\r 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), pentanucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs,\r the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the\r GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class,\r of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also\r found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were\r within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible\r primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the\r population structure among and within Chrysanthemum species."}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value_mlt": [{"interim": "Chrysanthemum"}]}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2020-05-05"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "Chrysanthemum SSR (Moe et al 2011).pdf", "filesize": [{"value": "1090 Kb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1090000.0, "url": {"url": "https://meral.edu.mm/record/49/files/Chrysanthemum SSR (Moe et al 2011).pdf"}, "version_id": "3447df1a-d3a0-4533-8b4d-218f8ec9c327"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{"subitem_journal_title": "Korean Journal of International Agriculture", "subitem_volume": "23"}]}, "item_1583103147082": {"attribute_name": "Conference papaers", "attribute_value_mlt": [{}]}, "item_1583103211336": {"attribute_name": "Books/reports/chapters", "attribute_value_mlt": [{}]}, "item_1583103233624": {"attribute_name": "Thesis/dissertations", "attribute_value_mlt": [{"subitem_supervisor(s)": []}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Moe Kyaw Thu"}, {"subitem_authors_fullname": "Sang-Bog Ra"}, {"subitem_authors_fullname": "Gi-An Lee"}, {"subitem_authors_fullname": "Myung-Chul Lee"}, {"subitem_authors_fullname": "Ha-Seung Park"}, {"subitem_authors_fullname": "Dong-Chan Kim"}, {"subitem_authors_fullname": "Cheol-Hwi Lee"}, {"subitem_authors_fullname": "Hyun-Gu Choi"}, {"subitem_authors_fullname": "Nak-Beom Jeon"}, {"subitem_authors_fullname": "Byung-Jun Choi"}, {"subitem_authors_fullname": "Ji-Youn Jung"}, {"subitem_authors_fullname": "Kyu-Min Lee"}, {"subitem_authors_fullname": "Yong-Jin Park"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Journal article"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2011"}, "item_1583159847033": {"attribute_name": "Identifier", "attribute_value": "https://yauor-yau.archive.knowledgearc.net/handle/123456789/70"}, "item_title": "Distribution and Frequencie of SSR Motifs in the Chrysanthemum SSR-enrich Library through 454 Pyrosequencing Technology", "item_type_id": "21", "owner": "1", "path": ["1582969313627"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000000049", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2020-03-05"}, "publish_date": "2020-03-05", "publish_status": "0", "recid": "49", "relation": {}, "relation_version_is_last": true, "title": ["Distribution and Frequencie of SSR Motifs in the Chrysanthemum SSR-enrich Library through 454 Pyrosequencing Technology"], "weko_shared_id": -1}