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  1. Yezin Agricultural University
  2. Department of Horticulture

In Vitro Regeneration of Selected Rice Genotypes (Oryza sativa L.) through Anther Culture

Permalink : http://hdl.handle.net/20.500.12678/0000000041
338a0b49-e1d8-4e25-8f37-a7375443953c
a569f65f-ec0d-4be8-a755-2ad05b572e7e
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92-98 92-98 Khin Soe Win.pdf (378.9 kB)
sha256: 83557f43999ad06f219d96510279e9587ba37b0a084854d8e5432913fe4a64d9
Publication type Journal article
Upload Type Publication
Title
In Vitro Regeneration of Selected Rice Genotypes (Oryza sativa L.) through Anther Culture
en
Publication date 2018-06
Authors
Khin Soe Win
Moe Kyaw Thu
Tin Tin Khaing
Htet Aung Htut
Khin Thida Myint
Description
Rice is one of the most important cereal crops in the world. Anther culture, one of the double hap-loid techniques, is simple and efficient for rice breeding. The present study was carried out to evaluate callus induction ability of the selected rice genotypes on the media supplemented with two different car-bon sources and to investigate suitable Benzylaminopurine (BAP) concentrations on plant regeneration of anther-derived calli. The selected 19 rice genotypes (14 indica and 5 tropical japonica) were used in the experiment. Anthers of each genotype were cultured on Chu (N6) medium supplemented with two types of carbon sources (4% maltose and 4% sucrose), 2mg.L-1 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.5 mg.L-1 kinetin for callus induction. Anther-derived calli were transferred to Murashige and Skoog (MS) medium supplemented with (0, 0.5, 1) mg.L-1 BAP, 1 mg.L-1 1-Naphthaleneacetic acid (NAA) and 1 mg.L-1 l kinetin for regeneration. The seventeen out of 19 rice genotypes produced callus on media supplemented with maltose while only 13 genotypes produced callus on media supplemented with sucrose due to genotype dependency. Tropical japonica had more callus induction from anther than indica among the tested genotypes. Callus induction varied from 0 to 19.22%. Paw San Taung Pyan Hmwe (tropical japonica) had the highest callus induction (19.22%) among the tested all. Yebaw Sein depicted the highest callus induction (6.78%) among the tested indica rice genotypes. Plant regeneration from callus varied from 0 to 25.15%. In tropical japonica genotypes, Bay Kyar Taung Pyan gave the highest value (25.15%) in green plants regeneration, while Hnan Kar gave the highest value (13.33%) among indica genotypes. In this experiment, both MS media supplemented with 0.5mg.L-1 BAP and 1mg.L-1 BAP showed maximum green plant regeneration although maximum green spot formation oc-curred only on media supplemented with 1mg.L-1 BAP.
Keywords
anther culture
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2020-05-05
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