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  1. Yadanabon University
  2. Department of Industrial Chemistry

Expression and Application of Peptide Materials Containg Functionalizing Sequence as a Metallothionein

http://hdl.handle.net/20.500.12678/0000000341
http://hdl.handle.net/20.500.12678/0000000341
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2b320ef6-4a7f-4eb9-be46-cac666f5846a
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Expression Expression and Application of Peptide.pdf (300 Kb)
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Journal article
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Title
Title Expression and Application of Peptide Materials Containg Functionalizing Sequence as a Metallothionein
Language en
Publication date 2007
Authors
Cho Lay Sint
Description
The present research focus on the peptides that are rich in cysteine or histidine amino acids, which are potential ligands for metal ions. These peptide sequence were designed from the nine kinds of metal binding fragments of xenopas laevies transcription factor III A zinc finger protein. The protein material was prepared by the rolling circle amplification (RCA) which was a useful and simple technique to amplify oligonucleotide for the biomaterials containing tandem repetitive sequence. The purification and characterization of protein were expressed in E.coli. This study targeted on the functionalized protein having the repetitive sequence of ( YICSFADCGAAYNKNWKLQAHLCKH )n as the RCA generated protein materials. The procedure for the purification of histidine tagged proteins has been developed using metal chelating affinity chromatography. This purification method can be used for proteins containing histidine tag and yielding high amounts of purified protein.
Keywords
protein design
Identifier https://oar.ydbu.edu.mm/handle/123456789/106
Journal articles
Journal of the Myanmar AcademyArts &Science
1
Conference papaers
Books/reports/chapters
Thesis/dissertations
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