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  1. Yadanabon University
  2. Department of Industrial Chemistry

Expression and Application of Peptide Materials Containg Functionalizing Sequence as a Metallothionein

Permalink : http://hdl.handle.net/20.500.12678/0000000341
f574f266-33fc-4ca5-8bbb-7a4adc89b9e3
2b320ef6-4a7f-4eb9-be46-cac666f5846a
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Expression Expression and Application of Peptide.pdf (300.4 kB)
sha256: 4056fa71aa54669030954355f55664676036f69c0f22cca9c10d7849f570bf67
Publication type Journal article
Upload Type Publication
Title
Expression and Application of Peptide Materials Containg Functionalizing Sequence as a Metallothionein
en
Publication date 2007
Authors
Cho Lay Sint
Description
The present research focus on the peptides that are rich in cysteine or histidine amino acids, which are potential ligands for metal ions. These peptide sequence were designed from the nine kinds of metal binding fragments of xenopas laevies transcription factor III A zinc finger protein. The protein material was prepared by the rolling circle amplification (RCA) which was a useful and simple technique to amplify oligonucleotide for the biomaterials containing tandem repetitive sequence. The purification and characterization of protein were expressed in E.coli. This study targeted on the functionalized protein having the repetitive sequence of ( YICSFADCGAAYNKNWKLQAHLCKH )n as the RCA generated protein materials. The procedure for the purification of histidine tagged proteins has been developed using metal chelating affinity chromatography. This purification method can be used for proteins containing histidine tag and yielding high amounts of purified protein.
Keywords
protein design
Files 300 Kb
Available
2020-05-05
application/pdf
preview
application/pdf
open_access
Expression and Application of Peptide.pdf

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