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  1. University of Yangon
  2. Department of Zoology

DNA Barcoding and Intra Species Analysis of the Ember Parrot Fish Scarus Rubroviolaceus using mtCO1

http://hdl.handle.net/20.500.12678/0000002698
http://hdl.handle.net/20.500.12678/0000002698
593dbe53-28e2-48ae-b3ef-01f187a6eda5
3df002f6-3d78-40e3-98fd-480e1e0436fa
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DNA DNA Barcoding and Intra Species Analysis.pdf (786 Kb)
Publication type
Journal article
Upload type
Publication
Title
Title DNA Barcoding and Intra Species Analysis of the Ember Parrot Fish Scarus Rubroviolaceus using mtCO1
Language en
Publication date 2016
Authors
Tiwary, Chandan
Haq, M.A.Badhul
Vaitheeswari, S
Kalaiselvi, M.
Sikder, Mohammad Nurul Azim
Wah Wah Min
Description
In this study S.rubroviolaceus form very ancient family group Scorpaenidae with structurally diverse group of subphylum Vertebrata was collected form from parangipettai (MAB06)for species identification with inter specific variation among barcoding region sequenceing cytochrome oxidase subunit I (COI) gene comparing with Australia (EF609452), Tonga (FJ584082), South Africa (GU805008), Iran (HQ149928). The intraspecific variation analysis in NCBI screend for COI sequence form different geographical regions results 99% to 100% identity. BOLD’s identification engine shows 99.68% to 100% similar of 20 specimens with conformation of COI species database tree that S.rubroviolaceus belongs to order Carangidae. The molar concentration of nucleotides in the COI region profiling barcoding valuates that high in thymine content are similar in all the samples, Cytosine as second predominant and guanine as least molar concentration. A helical wheel is ploted to illustrate the properties of alpha helices in proteins. Multiple sequence alignments for DNA sequences similarities query were analysed with Clustal W from EMBL tools.
Keywords
Interspecific variation
Identifier http://uyr.uy.edu.mm/handle/123456789/174
Journal articles
IRA-International Journal of Applied Sciences, Institute of Research Advances
5
Conference papaers
Books/reports/chapters
Thesis/dissertations
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