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  1. University of Yangon
  2. Department of Industrial Chemistry

Optimisation of Acid Hydrolysis of Grasses using Response Surface Methodology for the Preparation of Bioethanol

http://hdl.handle.net/20.500.12678/0000002326
http://hdl.handle.net/20.500.12678/0000002326
7d2c0892-9f93-4937-9cf1-929f77a44fb0
47efc859-72ca-4c4c-9224-b42674e78acd
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Optimisation Optimisation of Acid Hydrolysis of Grasses using Response.pdf (526 Kb)
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Journal article
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Title
Title Optimisation of Acid Hydrolysis of Grasses using Response Surface Methodology for the Preparation of Bioethanol
Language en
Publication date 2017
Authors
Soe Soe Than
Description
The grasses-Chloris barbata Sw. and Ischaemum pilosum Klein ex Willd were chosen as sources of lignocellulosic material for the preparation of ethanol. Fresh stems of grass were processed into fermentable sugars by acid hydrolysis using sulfuric acid. Optimisation of cellulose hydrolysis was performed by using Central Composite design of response surface methodology (RSM). Three variables such as acid concentration, acid volume and hydrolysing time were considered as influencing factors on the yield of fermentable sugars during acid hydrolysis. Baker’s yeast (Saccharomyces cerevisiae) was used in fermentation of the resulting
sugars under anaerobic condition. The maximum yields of ethanol by volume were 24.88 
0.20 % and 6.01  3.20 % with the yeast concentrations of 5 g/L and 4 g/L accordingly to their same reflux ratio of 1.01. Non-food plants of cellulosic materials become renewable feedstock for the production of ethanol. Cellulose in
cellulosic biomass is usually organised into microfibrils, containing up to 36 glucan chains having thousands of glucose residues. According to the degree of crystallinity, cellulose is classified into crystalline and amorphous cellulose. It can be hydrolytically broken down into glucose either enzymatically by cellulytic enzymes or chemically by sulfuric or other acids. A key advantage of acid pre-treatment is that a subsequent enzymatic hydrolysis step is sometimes not required, as the acid itself hydrolyses the biomass to yield fermentable sugars (Yu et al., 2010). Production of ethanol from cellulosic biomass contains three main processes, including pretreatment, hydrolysis, and fermentation. Pre-treatment facilitates the hydrolysis of cellulose to be rapid by altering the size and structure of biomass as well as its chemical composition. In the hydrolysis step, celluloses
are converted into monomer sugars. The resulting fermentable sugars could be fermented into ethanol by ethanol producing microorganisms, which can be either naturally occurring or genetically modified microorganisms (Zheng et al., 2009).
The present study investigated the ethanol opportunity from the grasses (Chloris barbata and Ischaemum pilosum) through acid hydrolysis followed by fermentation. Response surface methodology (RSM) was applied to optimise the process variables during cellulosic hydrolysis. Central composite design was chosen for experimental design and a second order polynomial equation was developed by using Design Expert 7 software (Stat-Ease Inc., 2007).
Keywords
Optimisation of Acid Hydrolysis
Identifier http://uyr.uy.edu.mm/handle/123456789/267
Journal articles
Chemical Engineering Transactions
56
Conference papaers
Books/reports/chapters
Thesis/dissertations
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