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Androgenesis of Selected Indica Rice (Oryza sativa L.) Genotypes and F1 Hybrids

http://hdl.handle.net/20.500.12678/0000000226
24d7d168-0666-4c29-8d90-e8cbf66d4c60
3cb061d2-90b4-46c8-bf88-ea1dc78f8e57
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hsu hsu yi mon MS thesis 2017.pdf (2820 Kb)
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Thesis
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Publication
Title
Title Androgenesis of Selected Indica Rice (Oryza sativa L.) Genotypes and F1 Hybrids
Language en
Publication date 2017-06
Authors
Hsu Yi Mon
Description
This experiment was carried out to evaluate the callusing ability and green
plant formation of selected indica rice genotypes and F1 progenies after crossing
selected genotypes. Shwe Man-1, Sin Akari-3, Sin Thwe Latt, Thee Htat Yin and
Yar-8 genotypes were selected as parental lines based on callusing ability of anther
culture. Five crosses (Shwe Man-1 x Thee Htat Yin, Thee Htat Yin x Sin Thwe Latt,
Sin Thwe Latt x Sin Akari-3, Yar-8 x Thee Htat Yin and Sin Thwe Latt x Yar-8) were
obtained by crossing the selected indica rice genotypes. Anthers of selected indica
rice genotypes and F1 hybrids were cultured on N6 medium with supplemented 2
mg·L-1 2,4-D and 0.5 mg·L-1 kinetin for callus induction. Calli were transferred onto
MS medium with 1 mg·L-1 IAA, 1 mg·L-1 NAA, 1 mg·L-1 BAP and 2 mg·L-1 kinetin.
All tested genotypes showed callus formation except one cross (Sin Thwe Latt
x Sin Akari-3) that showed no response on anther culture. Callus induction percentage
of responsive genotypes varied from 0.1-2.6%. Green plant formation from the
regenerated calli varied from 6.3-37.0%. Among parents, Yar-8 and Shwe Man-1
gave the highest callus induction and green plant formation. Cross involving Yar-8 as
female parent, Yar-8 x Thee Htat Yin increased callusing and green plant formation
ability. These three genotypes (Yar-8, Shwe Man-1 and cross of Yar-8 x Thee Htat
Yin) produced green plants. The anther-derived plants of these three genotypes were
haploid and double haploid. According to the results, Yar-8 genotype has the potential
for double haploid production through anther culture in rice breeding program.
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/264
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