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This experiment was conducted with three objectives; (1) to observe the effects of different explants on in vitro shoot induction of asparagus, (2) to determine the optimum concentrations of 6-benzyladeno-purine (BAP) for shoot multiplication of asparagus, and (3) to compare the single shoots and cluster shoots for root induction of asparagus. Three different explant types; apical bud, lateral bud and spear segment were cultured onto MS medium to induce shoots in initial culture. Shoot tips derived from apical and lateral buds of initial culture were sub-cultured onto MS medium with 0.5 mg. L-1 1-naphthaleneacetic acid (NAA) and 0, 2, 3 and 4 mg. L-1 6-benzyladeno-purine (BAP) for shoot multiplication stage. After multiplication, two types of shoots (single and cluster shoots) were cultured onto MS medium with 1.5 and 2.5 mg. L-1 indole-3-butyric acid (IBA) for root induction.\r Shoot formation of three different explant types was observed approximately 20 days after culture. For initial culture, apical bud gave 63 % of shoot formation followed by lateral bud (44 %) and spear segment (20 %) respectively. The maximum number of shoots (3 shoots per explant) was observed in apical bud. Although the maximum number of nodes (2 nodes per shoot) was found in apical and lateral buds, lateral bud gave longer shoot length (2.6 cm). The medium supplement with 2 mg. L-1 BAP gave the highest number of shoots (9 shoots per explant), shoot length (2 cm), and number of nodes (2 nodes per shoot) in multiplication stage. Cluster shoots produced higher root formation percentage in both levels of IBA concentrations than single shoot. Among two different IBA levels, 2.5 mg. L-1 IBA gave higher root formation percentage than 1.5 mg. L-1 IBA. Based on this research finding, apical bud is a suitable explant type for shoot formation. The medium supplement with 2 mg. L-1 BAP could be selected to get maximum number of shoots per explant, nodes per shoot and longest shoot length. It was observed that cluster shoots formed roots well in 2.5 mg. L-1 IBA containing medium. 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  1. Yezin Agricultural University
  2. Department of Horticulture

EFFECTS OF DIFFERENT EXPLANT TYPES, PLANT GROWTH REGULATORS AND SHOOT DENSITY ON IN VITRO REGENERATION OF ASPARAGUS (Asparagus officinallis L.)

Permalink : http://hdl.handle.net/20.500.12678/0000000210
fbf4fd04-8b76-4664-ad10-e81c3cd78a46
32f5ffa7-2b2d-44f2-a08f-e66202d6f449
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maung maung maung MS thesis 2018.pdf (2.8 MB)
sha256: 867ffc02608a36e1949a8644c52f281856a4c7252bfc7380d9b6e6effb66db81
Publication type Thesis
Upload Type Publication
Title
EFFECTS OF DIFFERENT EXPLANT TYPES, PLANT GROWTH REGULATORS AND SHOOT DENSITY ON IN VITRO REGENERATION OF ASPARAGUS (Asparagus officinallis L.)
en
Publication date 2018-02
Authors
Maung Maung
Description
Asparagus are grown throughout the world. The most economically important Asparagus species is garden Asparagus (Asparagus officinallis L.). It is a kind of health caring vegetables contributing distinctive taste and an abundant source of various nutrients. The production of Asparagus species is increasing and becoming popular. This experiment was conducted with three objectives; (1) to observe the effects of different explants on in vitro shoot induction of asparagus, (2) to determine the optimum concentrations of 6-benzyladeno-purine (BAP) for shoot multiplication of asparagus, and (3) to compare the single shoots and cluster shoots for root induction of asparagus. Three different explant types; apical bud, lateral bud and spear segment were cultured onto MS medium to induce shoots in initial culture. Shoot tips derived from apical and lateral buds of initial culture were sub-cultured onto MS medium with 0.5 mg. L-1 1-naphthaleneacetic acid (NAA) and 0, 2, 3 and 4 mg. L-1 6-benzyladeno-purine (BAP) for shoot multiplication stage. After multiplication, two types of shoots (single and cluster shoots) were cultured onto MS medium with 1.5 and 2.5 mg. L-1 indole-3-butyric acid (IBA) for root induction. Shoot formation of three different explant types was observed approximately 20 days after culture. For initial culture, apical bud gave 63 % of shoot formation followed by lateral bud (44 %) and spear segment (20 %) respectively. The maximum number of shoots (3 shoots per explant) was observed in apical bud. Although the maximum number of nodes (2 nodes per shoot) was found in apical and lateral buds, lateral bud gave longer shoot length (2.6 cm). The medium supplement with 2 mg. L-1 BAP gave the highest number of shoots (9 shoots per explant), shoot length (2 cm), and number of nodes (2 nodes per shoot) in multiplication stage. Cluster shoots produced higher root formation percentage in both levels of IBA concentrations than single shoot. Among two different IBA levels, 2.5 mg. L-1 IBA gave higher root formation percentage than 1.5 mg. L-1 IBA. Based on this research finding, apical bud is a suitable explant type for shoot formation. The medium supplement with 2 mg. L-1 BAP could be selected to get maximum number of shoots per explant, nodes per shoot and longest shoot length. It was observed that cluster shoots formed roots well in 2.5 mg. L-1 IBA containing medium. Cluster shoots have potential for rooting of in vitro developing shoots rather than single shoots.
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