{"_buckets": {"deposit": "8eef7a7d-e3e9-4635-bb9e-f7cb5548b948"}, "_deposit": {"id": "189", "owners": [], "pid": {"revision_id": 0, "type": "recid", "value": "189"}, "status": "published"}, "_oai": {"id": "oai:meral.edu.mm:recid/189", "sets": ["user-yau"]}, "communities": ["yau"], "control_number": "189", "item_1583103067471": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Effects of Plant Growth Regulators and Culture Systems on In Vitro Regeneration and Microtuberization of Meristem and Shoot Tip Culture of Selected Potato Cultivars", "subitem_1551255648112": "en"}]}, "item_1583103085720": {"attribute_name": "Description", "attribute_value_mlt": [{"interim": "The experiment was conducted at the Plant Tissue Culture Laboratory,\r Department of Horticulture and Agricultural Biotechnology, YAU from 2015 to 2016.\r Three selected potato cultivars, Up-to-date, L-11 and Atlantic were used in this study. In\r initial culture, RCB design was used with 4 replications and treatments were three potato\r cultivars. There were two main experiments in this study: meristem culture and shoot tip\r culture.\r In experiment I, two factors factorial arrangement in RCB design was used with\r 4 replications. The meristem explants, were used for shoots induction in three potato\r cultivars as factor A, using 9 different combinations of 6-benzyl aminopurine (BAP) 0.0,\r 1.5, 3.0 mg.L-1 and gibberellic acid (GA3) 0.0, 0.3, 0.6 mg.L-1 as factor B. The meristem\r explants produced hundred percent survival shoots in medium both supplemented with\r 1.5 mg.L-1BAP in combination with 0.6 mg.L-1GA3 and 3.0 mg.L-1BAP in combination\r with 0.3 mg.L-1GA3 for all tested cultivars. From this experiment, the shoots induced by\r Up-to-date were used for shoot multiplication due to better response in meristem culture.\r For shoot multiplication, three different combinations of plant growth regulators (PGRs)\r 0.05 mg.L-1Naphthalene acetic acid (NAA) in combinations with two levels of 1.0 and\r 0.5 mg.L-1BAP, and PGRs free as control were used as treatments in 3 x 4 RCB design.\r Among all treatments the significantly longest shoot length was observed in shoots\r developed on PGRs free medium. Microtuberization was done to evaluate the effects of\r two different concentrations of sucrose 6%, 8% and two level of BAP 3 mg.L-1, 5 mg.L-1\r using RCB design with 4 replications. Plantlets developed on medium supplemented with\r 8% sucrose and 3 mg.L-1BAP showed 100% microtuberization and gave the largest sized\r tuber in meristem culture derived explants.\r In experiment II, shoot multiplication of shoot tip culture derived three potato\r cultivars, 3 factor factorial in RCB arrangement was used with 4 replications, factor A\r was three potato cultivars, factor B was 3 different combinations of PGRs used in\r experiment I and factor C was 2 culture systems: solid and liquid culture. Liquid culture\r system was superior to solid MS in such growth parameter as shoot length, no. of nodes,\r no. of roots and fresh weight. PGRs free provided the best result in liquid medium.\r However, 0.05 mg.L-1NAA and 0.5 mg.L-1BAP combination was suitable to use in solid\r medium. For microtuberization of shoots derived from shoot tip culture, the same\r treatments of sucrose and PGRs applied in experiment I were used in three potato cultivars. L-11 cultivar provided 100% microtuberization in medium supplemented with 8\r % sucrose and 5 mg.L-1BAP. Medium modified with 8% sucrose and 3 mg.L-1BAP\r combinations gave 96.43% microtuberization in L-11 and 92.85% Up-to-date cultivar. In\r comparing meristem culture with shoot tip culture in Up-to-date cultivar, meristem\r explants provided better performance in shoot multiplication, microtuberization and size\r of microtuber. In confirmation of plantlets developed from meristem culture by using\r “POCKET DIAGNOSTIC”, these plantlets were free from potato five multivirus and\r Rhizotonia solanacearum."}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value_mlt": [{"interim": "PGRs"}]}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2020-05-05"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "Khaing Khaing Oo(HSC-64) MS Thesis 2016.pdf", "filesize": [{"value": "6845 Kb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 6845000.0, "url": {"url": "https://meral.edu.mm/record/189/files/Khaing Khaing Oo(HSC-64) MS Thesis 2016.pdf"}, "version_id": "e498d8a8-1dab-43bc-b3e0-c5a1e64dd28a"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{}]}, "item_1583103147082": {"attribute_name": "Conference papaers", "attribute_value_mlt": [{}]}, "item_1583103211336": {"attribute_name": "Books/reports/chapters", "attribute_value_mlt": [{}]}, "item_1583103233624": {"attribute_name": "Thesis/dissertations", "attribute_value_mlt": [{"subitem_supervisor(s)": []}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Khaing Khaing Oo"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Thesis"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2016-11"}, "item_1583159847033": {"attribute_name": "Identifier", "attribute_value": "https://yauor-yau.archive.knowledgearc.net/handle/123456789/265"}, "item_title": "Effects of Plant Growth Regulators and Culture Systems on In Vitro Regeneration and Microtuberization of Meristem and Shoot Tip Culture of Selected Potato Cultivars", "item_type_id": "21", "owner": "1", "path": ["1582969222431"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000000189", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2020-03-05"}, "publish_date": "2020-03-05", "publish_status": "0", "recid": "189", "relation": {}, "relation_version_is_last": true, "title": ["Effects of Plant Growth Regulators and Culture Systems on In Vitro Regeneration and Microtuberization of Meristem and Shoot Tip Culture of Selected Potato Cultivars"], "weko_shared_id": -1}