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Item

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Sclerotium rolfsii Sacc. isolated from ten host plants, chickpea, chilli, cowpea, eggplant, groundnut, sesame, soybean, sunflower, taro and tomato were studied for their variation based on morphological characters of mycelium, sclerotial characters, and mycelial compatibility grouping. The test was arranged as completely randomized design (CRD) with five replications. It was observed that most of the isolates produced fluffy colonies while the colony of chickpea isolate was less fluffy on potato dextrose agar (PDA) media. All ten isolates were the fast growing fungi since their growth rates ranged from 27 to 30 mm in diameter day-1 on PDA media. The maximum number of sclerotia at 28 days after incubation, i.e., 1018, was produced by the sesame isolate while the minimum number, 281, by the chickpea isolate. 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Variation in Morphological Characteristics of Sclerotium rolfsii and Biocontrol Efficacy of Trichoderma harzianum on This Fungus

http://hdl.handle.net/20.500.12678/0000000167
57b29b59-3a04-4408-9bbc-52d0cf682698
60537c56-474b-4d3e-b52d-c279096019ff
None
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Nay Nay Nay Oo_Thesis.pdf (1353 Kb)
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Thesis
Upload type
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Title
Title Variation in Morphological Characteristics of Sclerotium rolfsii and Biocontrol Efficacy of Trichoderma harzianum on This Fungus
Language en
Publication date 2015-11
Authors
Oo, Nay Nay
Description
Three experiments were carried out at the Department of Plant Pathology, Yezin Agricultural University during 2013-2015. Sclerotium rolfsii Sacc. isolated from ten host plants, chickpea, chilli, cowpea, eggplant, groundnut, sesame, soybean, sunflower, taro and tomato were studied for their variation based on morphological characters of mycelium, sclerotial characters, and mycelial compatibility grouping. The test was arranged as completely randomized design (CRD) with five replications. It was observed that most of the isolates produced fluffy colonies while the colony of chickpea isolate was less fluffy on potato dextrose agar (PDA) media. All ten isolates were the fast growing fungi since their growth rates ranged from 27 to 30 mm in diameter day-1 on PDA media. The maximum number of sclerotia at 28 days after incubation, i.e., 1018, was produced by the sesame isolate while the minimum number, 281, by the chickpea isolate. Based on mycelial compatibility groupings (MCGs) test, only two parings, the cowpea and taro isolates that designated as MCG 3 and the groundnut and sunflower isolates that designated as MCG 5, were compatible. As a result, eight different mycelial compatibility groups (MCG 1 to 8) were identified among them. The second experiment was carried out to study the biocontrol efficacy of Trichoderma harzianum on mycelial growth of the eight isolates on PDA media and the experiment was arranged as CRD with five replications. The inhibition efficacy of T. harzianum was determined by dual culture technique in vitro. The highest inhibition percent (68%) of T. harzianum was found against the sesame isolate. The third experiment was carried out to study the cross-pathogenic ability of eight isolates on different hosts and percent disease control of T. harzianum on these crops. The experiment was arranged as three factors factorial; Factor A - isolates, Factor B - with or without T. harzianum, Factor C - crops, with three replications. The results revealed that all the isolates had cross pathogenesis to all tested crops. However, the chickpea isolate was low pathogenic among them. It was also observed that there was no disease control of T. harzianum on the chickpea isolate. The percent disease control of T. harzianum on the other seven isolates of S. rolfsii ranged from 6% to 30%.
Keywords
Sclerotium rolfsii
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/305
Journal articles
Yezin Agricultural University
Conference papaers
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Thesis/dissertations
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