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Effects of Different Explant Types, Plant Growth Regulators and Shoot Density on In Vitro Regeneration of Asparagus (Asparagus officinallis L.)

http://hdl.handle.net/20.500.12678/0000000162
fb99330c-9b52-48ec-90a0-958d0f741fec
a080db7b-cdef-4bef-b676-c2276d65b12a
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14 14 Mg Mg 109-115.pdf (1148 Kb)
Publication type
Journal article
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Publication
Title
Title Effects of Different Explant Types, Plant Growth Regulators and Shoot Density on In Vitro Regeneration of Asparagus (Asparagus officinallis L.)
Language en
Publication date 2019-01
Authors
Maung Maung
Khin Thida Myint
Moe Kyaw Thu
Description
The asparagus plants known as Ka-nyut in Myanmar are commercially cultivated in many places of central lowlands and eastern mountainous regions. This experiment was carried out to observe the ef-fects of different explants on in vitro shoot induction, to verify the optimum concentrations of 6-benzyladeno-purine (BAP) for shoot multiplication, and to compare the behaviours of the single shoots and cluster shoots for root induction of asparagus. Three different explant types – apical bud, lateral bud and spear segment – were cultured onto MS medium to induce shoots in initial culture. Shoot formation of three different explant types was observed around 20 days after culture. For initial culture, apical bud gave 63 % of shoot formation followed by lateral bud (44 %) and spear segment (20 %) respectively. Shoot tips derived from apical and lateral buds of initial culture were sub-cultured onto MS medium with 0.5 mg. L-1 of 1-naphthaleneacetic acid (NAA) and 0, 2, 3 or 4 mg. L-1 of BAP for shoot multiplica-tion. The medium supplemented with 2 mg. L-1 of BAP gave the highest number of shoots (9 shoots per explant), the longest shoot length (2 cm), and the highest number of nodes (2 nodes per shoot). After shoot multiplication, two types of shoots (single and cluster shoots) were cultured again onto MS medi-um with 1.5 and 2.5 mg. L-1 of indole-3-butyric acid (IBA) for root induction. It was observed that clus-ter shoots formed roots well in 2.5 mg. L-1 IBA-containing medium. Cluster shoots have more potential for rooting of in vitro developing shoots than single shoots do.
Keywords
shoot multiplication, root induction, asparagus
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/239
Journal articles
Ywetsaine Sarpay
6
Conference papaers
Books/reports/chapters
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0
0
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