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Item

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  1. Yezin Agricultural University
  2. Department of Plant Pathology

Detection of Blast Resistance genes on Cultivated Rice in Myanmar using SSR and InDel Markers

http://hdl.handle.net/20.500.12678/0000000160
http://hdl.handle.net/20.500.12678/0000000160
d4665aa8-1567-4541-a695-796ae68cabf9
41088cb8-bc13-49cf-b6ff-4457ce14b129
None
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Thet Thet Yee Khaing_Journal_ (P.PP-9).pdf (5914 Kb)
Publication type
Journal article
Upload type
Publication
Title
Title Detection of Blast Resistance genes on Cultivated Rice in Myanmar using SSR and InDel Markers
Language en
Publication date 2019-12
Authors
Khaing, Thet Yee
Win, Nang Kyu Kyu
Win, Kyaw Kyaw
Htun, Than Myint
Thein, Min San
Naing, Tin Aye Aye
Description
The present study was conducted to detect the presence of blast resistance genes in cultivated rice in Myanmar. Thirteen blast resistance genes _Pib, Pik, Pik-h, Pik-m, Pik-s,
Pi7, Pish, Pita, Pita-2, Piz-t, Pi(5)t and Piz-5_were screened on 57 released varieties by
using 13 linked markers; 12 Simple Sequence Repeats and one Insertion Deletion, through
polymerase chain reaction based methods. The genetic frequencies of these 13 major blast
resistance genes ranged from 43.86% (Pik-m) to 7.02% (Piz-t) whereas Pita resistance gene amplicon was not observed on all tested varieties. The Pik-m gene was detected as the most prevalent one amongst the genotypes followed by Pi7 which distributed in frequency of 42.11%, Pik , Pita-2 and Pi5(t) (22.81%), Pik-s (21.05%) , Pib and Pik-h (19.30%), Pik (17.54%), Piz-5 (14.04%) and Pish (12.28%). The genotypic variation between the released varieties was detected. Among 57 released varieties, two varieties — Manawthuka and Mote Soe Ma Kyway Pyay line MMK 03-23-3 — possessed seven blast resistance genes the other 12 varieties carried single genes, five varieties five resistance genes, 13 varieties four resistance genes, six varieties three resistance genes and 15 varieties two resistance genes. In the rest four varieties resistance genes could not be amplified by tested markers. This study provided the information of resistant varieties that will be beneficial in pre-breeding program for developing of rice blast resistance varieties.
Keywords
blast, insertion deletion, resistance gene, rice, simple sequence repeats
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/235
Journal articles
Yewtsaine Sarpay
Conference papaers
Books/reports/chapters
Thesis/dissertations
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