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Sequence Information on Simple Sequence Repeat and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

http://hdl.handle.net/20.500.12678/0000000146
b968caf4-7806-462a-95d5-b851f8ef61db
0c12d149-8131-4b20-a814-414c482e2a34
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Mungbean Mungbean Transcriptome (Moe et al.2011).pdf (714 Kb)
Publication type
Journal article
Upload type
Publication
Title
Title Sequence Information on Simple Sequence Repeat and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean
Language en
Publication date 2011
Authors
Moe Kyaw Thu
Jong-Wook Chung
Young-Il Cho
Jung Kyaung Moon
Ja-Hwan Ku
Jin-Kyo Jung
Jungran Lee
Yong-Jin Park
Description
Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen,
with early maturity, and relatively good drought resistance. We used 454 sequencing technology for
transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs
(≥500 bp) with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional
annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher
number of simple sequence repeat (SSR) motifs was identified in Jangan (1 630) compared with that of
Sunhwa (1 334). A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single
nucleotide polymorphisms (SNPs) and indels with 2 098 high-confidence candidates were identified in
the two mungbean varieties. The average distance between individual SNPs was ∼860 bp. Our report
demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement
programs.
Keywords
SSR
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/55
Journal articles
Journal of Integrative Plant Biology
Conference papaers
Books/reports/chapters
Thesis/dissertations
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0
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