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Item
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Sequence Information on Simple Sequence Repeat and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean
http://hdl.handle.net/20.500.12678/0000000146
http://hdl.handle.net/20.500.12678/0000000146b968caf4-7806-462a-95d5-b851f8ef61db
0c12d149-8131-4b20-a814-414c482e2a34
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Mungbean Transcriptome (Moe et al.2011).pdf (714 Kb)
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Journal article | ||||||
Upload type | ||||||
Publication | ||||||
Title | ||||||
Title | Sequence Information on Simple Sequence Repeat and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean | |||||
Language | en | |||||
Publication date | 2011 | |||||
Authors | ||||||
Moe Kyaw Thu | ||||||
Jong-Wook Chung | ||||||
Young-Il Cho | ||||||
Jung Kyaung Moon | ||||||
Ja-Hwan Ku | ||||||
Jin-Kyo Jung | ||||||
Jungran Lee | ||||||
Yong-Jin Park | ||||||
Description | ||||||
Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs (≥500 bp) with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat (SSR) motifs was identified in Jangan (1 630) compared with that of Sunhwa (1 334). A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms (SNPs) and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was ∼860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs. |
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Keywords | ||||||
SSR | ||||||
Identifier | https://yauor-yau.archive.knowledgearc.net/handle/123456789/55 | |||||
Journal articles | ||||||
Journal of Integrative Plant Biology | ||||||
Conference papaers | ||||||
Books/reports/chapters | ||||||
Thesis/dissertations |