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Item

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Effects of Explants and Growth Regulators on In Vitro Regeneration of Dragon Fruit (Hylocereus undatus Haworth)

http://hdl.handle.net/20.500.12678/0000000118
90a0eb46-5c96-4bfc-bfc9-c669fdb445c1
3865faf9-b64e-4a99-a5bc-dbe009ee1837
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soe soe thiha MS thesis 2019.pdf (573 Kb)
Publication type
Thesis
Upload type
Publication
Title
Title Effects of Explants and Growth Regulators on In Vitro Regeneration of Dragon Fruit (Hylocereus undatus Haworth)
Language en
Publication date 2019-02
Authors
Soe Thiha
Description
The experiment was conducted at Plant Tissue Culture Laboratory, Experiment
and Lecture Building - 2 (ELB-2), Department of Horticulture, Yezin Agricultural
University (YAU) from 2016 to 2018. This study was carried out to investigate effects of
explant types and to examine effects of different levels of plant growth regulators on
in vitro regeneration of dragon fruit. The experiment was arranged in factorial RCB
design with four replications and factor A was two kinds of explants; distal and proximal
portions (7-10 mm) of shoot developed in vitro and factor B was five different levels of
N-6 benzyl aminopurine (BAP) for shoot multiplication. For root induction, factor A was
two types of explants; distal and proximal portions (7-10 mm) and factor B was three
different levels of naphthalene acetic acid (NAA).
According to the experimental results, in shoot multiplication, the maximum
number of shoots per explant (4.42) and the longest shoot (1.39 cm) was obtained from
proximal portion of shoot explants. The medium supplemented with 10 μM BAP was
observed production of the highest number of shoots, shoot length and shoot fresh weight.
In root induction, there was no significant effect of different explant types on number of
roots, root length and fresh weight of plantlets. However, the maximum number of roots,
root length and fresh weight of plantlets were found at 0.3 μM NAA containing media.
By the results, it can be concluded that the proximal portion of shoot explant should be
used in shoot multiplication and the medium containing 10 μM BAP is recommended.
For root induction, the explants cultured on 0.3μM NAA containing medium is
recommended for in vitro root induction stage.
Keywords
BAP
Identifier https://yauor-yau.archive.knowledgearc.net/handle/123456789/276
Journal articles
Conference papaers
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Thesis/dissertations
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