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  1. University of Mandalay
  2. Department of Zoology

Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis)

http://hdl.handle.net/20.500.12678/0000001011
http://hdl.handle.net/20.500.12678/0000001011
b50fae6c-2007-4827-aed2-ad6a373a73c0
ba56df57-f3a8-44a1-b603-71c82cd38b83
Publication type
Journal article
Upload type
Publication
Title
Title Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis)
Language en
Publication date 2010
Authors
Aung Kyaw Swar Oo
Kaneko, Gen
Hirayama, Makoto
Kinoshita, Shigeharu
Watabe, Shugo
Description
A monogonont rotifer Brachionus plicatilis has been widely used as a model organism for physiological, ecological studies and for ecotoxicology. Because of the availability of parthenogenetic mode of reproduction as well as its versatility to be used as live food in aquaculture, the population dynamic studies using the rotifer have become more important and acquired the priority over those using other species. Although many studies have been conducted to identify environmental factors that influence rotifer populations, the molecular mechanisms involved still remain to be elucidated. In this study, gene(s) differentially expressed by calorie restriction in the rotifer was analyzed, where a calorie-restricted group was fed 3h day-1 and a well-fed group fed ad libitum. A subtracted cDNA library from the calorie-restricted rotifer was con-structed using suppression subtractive hybridization (SSH). One hundred sixty-three expressed sequence tags (ESTs) were identiWed, which included 109 putative genes with a high identity to known genes in the publicly available database as well as 54 unknown ESTs. After assembling, a total of 38 different genes were obtained among 109 ESTs. Further validation of expression by semi-quantitative reverse transcription-PCR showed that 29 out of the 38 genes obtained by SSH were up regulated by calorie restriction.
Keywords
Monogonont rotifer
Identifier doi:10.1007/s00360-009-0389-6
Journal articles
1
Journal of Comparative Physiology B
180
Conference papaers
Books/reports/chapters
Thesis/dissertations
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