{"created":"2024-02-12T08:31:20.794567+00:00","id":9295,"links":{},"metadata":{"_buckets":{"deposit":"a7139c52-8f3c-44ed-8b21-f87c539d5b61"},"_deposit":{"created_by":12,"id":"9295","owner":"12","owners":[12],"owners_ext":{"displayname":"","email":"hlaingyu2010@gmail.com","username":""},"pid":{"revision_id":0,"type":"depid","value":"9295"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/00009295","sets":["1582963390870:1582967769683"]},"author_link":[],"item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Comparison of Three DNA Extraction Methods from the Leaves of Capsicum annuum L. (Nga-yoke)","subitem_1551255648112":"en"}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"This study is focused on comparison of plant genomic DNA extraction from Capsicum annuum L. The molecular technique is another highly reliable alternative for plant species identification besides phytochemical profiling. The DNA were extracted from the tender leaves of C. annuum L. by using three different methods such as CTAB, Dellaporta and SDS methods to determine the most cost-effective and time efficient method to extract DNA. Then, the quality of extracted genomic DNA was amplified Polymerase Chain Reaction (PCR) and checked by gel electrophoresis and NanoDrop spectrophotometer.The result of NanoDrop spectrophotometer revealed that DNA from modified CTAB method yielded 841.7 ng/μL concentration with A260/A280 ratio of 1.99 and with A260/A230 ratio of 1.34. That isolated DNA has no protein although a little others impurities contaminations. The gel electrophoresis of modified CTAB method gave the intact DNA band and PCR had the expected band (~500 bp). Among three extraction methods, the CTAB method with little modifications was superior in the amount and purity of DNA compared to the Dellaporta and SDS methods."}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value_mlt":[{"interim":"DNA"},{"interim":"PCR"},{"interim":"NanoDrop spectrophotometer"},{"interim":"Capiscum annuum L."},{"interim":"CTAB"}]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-12"}],"displaytype":"preview","filename":"Yoon Myat Noe (235 to 242).pdf","filesize":[{"value":"208 KB"}],"format":"application/pdf","licensetype":"license_0","url":{"url":"https://meral.edu.mm/record/9295/files/Yoon Myat Noe (235 to 242).pdf"},"version_id":"df813a70-8d07-4df4-8391-46da4d3137cc"}]},"item_1583103131163":{"attribute_name":"Journal articles","attribute_value_mlt":[{"subitem_issue":"2022","subitem_journal_title":"University of Yangon Research Journal","subitem_pages":"235-242","subitem_volume":"11, No. 1"}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Yoon Myat Noe"},{"subitem_authors_fullname":"Swe Swe Aye"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Journal article"}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2022-12-31"},"item_title":"Comparison of Three DNA Extraction Methods from the Leaves of Capsicum annuum L. (Nga-yoke)","item_type_id":"21","owner":"12","path":["1582967769683"],"publish_date":"2024-02-12","publish_status":"0","recid":"9295","relation_version_is_last":true,"title":["Comparison of Three DNA Extraction Methods from the Leaves of Capsicum annuum L. (Nga-yoke)"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-12T08:37:48.209678+00:00"}