{"_buckets": {"deposit": "54ba848a-d0ca-49a1-8bc1-4666a16c2181"}, "_deposit": {"created_by": 30, "id": "5326", "owner": "30", "owners": [30], "owners_ext": {"displayname": "", "username": ""}, "pid": {"revision_id": 0, "type": "recid", "value": "5326"}, "status": "published"}, "_oai": {"id": "oai:meral.edu.mm:recid/5326", "sets": ["user-ytu"]}, "communities": ["ytu"], "item_1583103067471": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Influence of an external electric field on removal of protein fouling on a stainless steel surface by proteolytic enzymes", "subitem_1551255648112": "en"}]}, "item_1583103085720": {"attribute_name": "Description", "attribute_value_mlt": [{"interim": "Enzymatic cleaning is a potentially useful method for removing proteinaceous fouling from solid surfaces\nunder mild conditions. Herein, the influence of an external electric field on the enzymatic cleaning of a\nmetal surface fouled with a protein was investigated. The model fouling protein (BSA; lysozyme) was\nprepared on a stainless steel (St) surface, and the resulting surface subjected to enzymatic cleaning\nwith an electric potential being applied to the St plate. Trypsin, \u0002-chymotrypsin, and thermolysin were\nused as model proteases. The amounts of protein remaining on the plate before and during the cleaning\nprocess were measured by means of a reflection absorption technique using Fourier transform infrared\nspectroscopy. In the case for BSA fouling, the cleaning efficiency of the protease tended to increase at more\nnegative applied potentials. Whereas, there was an optimum applied potential for removing the lysozyme\nfouling. Atomic force microscopy analyses indicated that applying an adequate range of electric potential\nenhanced the enzymatic removal of protein fouling inside scratches on the St plate surface. These findings\nsuggest the existence of two modes of electrostatic interactions for the external electric field, one with\nprotease molecules and the other with digested fragments of the fouling protein."}]}, "item_1583103108160": {"attribute_name": "Keywords", "attribute_value_mlt": [{"interim": "Enzymatic cleaning"}, {"interim": "Electric potential"}, {"interim": "Protein fouling"}, {"interim": "Stainless steel"}, {"interim": "Atomic force microscopy"}]}, "item_1583103120197": {"attribute_name": "Files", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2020-09-10"}], "displaytype": "preview", "download_preview_message": "", "file_order": 0, "filename": "Influence of an external electric field on removal of protein fouling on.pdf", "filesize": [{"value": "2 Mb"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_0", "mimetype": "application/pdf", "size": 2000000.0, "url": {"url": "https://meral.edu.mm/record/5326/files/Influence of an external electric field on removal of protein fouling on.pdf"}, "version_id": "3ad74298-cd29-4831-9c73-f3d6f1a38445"}]}, "item_1583103131163": {"attribute_name": "Journal articles", "attribute_value_mlt": [{"subitem_journal_title": "Colloids and Surfaces B: Biointerfaces", "subitem_pages": "pp 118_ 124", "subitem_volume": "159"}]}, "item_1583105942107": {"attribute_name": "Authors", "attribute_value_mlt": [{"subitem_authors": [{"subitem_authors_fullname": "Ei Ei Htwe"}, {"subitem_authors_fullname": "Yuhi Nakama"}, {"subitem_authors_fullname": "Hiroyuki Imanaka"}, {"subitem_authors_fullname": "Naoyuki Ishida"}, {"subitem_authors_fullname": "Koreyoshi Imamura"}]}]}, "item_1583108359239": {"attribute_name": "Upload type", "attribute_value_mlt": [{"interim": "Publication"}]}, "item_1583108428133": {"attribute_name": "Publication type", "attribute_value_mlt": [{"interim": "Journal article"}]}, "item_1583159729339": {"attribute_name": "Publication date", "attribute_value": "2017-11-27"}, "item_title": "Influence of an external electric field on removal of protein fouling on a stainless steel surface by proteolytic enzymes", "item_type_id": "21", "owner": "30", "path": ["1596105561292"], "permalink_uri": "http://hdl.handle.net/20.500.12678/0000005326", "pubdate": {"attribute_name": "Deposited date", "attribute_value": "2020-09-10"}, "publish_date": "2020-09-10", "publish_status": "0", "recid": "5326", "relation": {}, "relation_version_is_last": true, "title": ["Influence of an external electric field on removal of protein fouling on a stainless steel surface by proteolytic enzymes"], "weko_shared_id": -1}