{"created":"2020-03-08T04:59:36.094395+00:00","id":40,"links":{},"metadata":{"_buckets":{"deposit":"d265e8c6-33bf-4dae-a20b-adc453649fb1"},"_deposit":{"id":"40","owners":[],"pid":{"revision_id":0,"type":"recid","value":"40"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/40","sets":["1582963567848:1582969222431"]},"communities":["yau"],"control_number":"40","item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"In Vitro Propagation of Red Banana Musa acuminata (AAA) cv. Red Dacca","subitem_1551255648112":"en"}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"The experiments were carried out at the Plant Tissue Culture Laboratory, Department of Horticulture and Agricultural Biotechnology, Yezin Agricultural University from June 2011 to July 2012, in order to investigate the optimum plant growth regulators (PGRs) concentrations for different stages in vitro propagation of red banana Musa acuminata (AAA) cv. Red Dacca. Two experiments were employed in randomized complete block design (RCBD). Murashige and Skoog (MS) 1962 was used as basal medium throughout the study.\r In both initial culture and multiplication stages (experiment 1), the examination of the effect of different cytokinins application on multiple shoot formation was conducted. The treatments were four levels of 6-benzylamino purine (BAP) (2.5, 5.0, 7.5, 10.0 mg· L-1) and three levels of Thidiazuron (TDZ) (0.05, 0.15, 0.25 mg· L-1). In initial culture stage, the explants were inoculated in liquid medium with cotton culture for 2 weeks, then transferred to semi-solid medium for 6 weeks and incubated at 25 ± 2°C under 16 hrs photoperiod of 25 μmolm־²s־¹ light intensity. In multiplication stage, shoots resulted from initial culture were treated with TDZ 0.15 mg· L-1 in two subcultures (S1, S2) and the cultures were incubated in continuous darkness condition throughout the 6 weeks period of each subculture. Survival percent, normal shoot formation percent and days to induce new shoot were collected for both culture stages. The data on number of shoots, shoot length and shoot fresh weight were collected after each subculture. In rooting stage (experiment 2), six PGRs treatments (0.5 and 1.0 mg· L-1 each of NAA and IBA, 0.5 and 1.0 mg· L-1 of NAA in combination with 0.5 mg· L-1 IBA) were given in half MS medium. The PGRs treatments were given in two ways for rooting. One group was cultured on PGRs free medium for 2 weeks prior to transfer to rooting medium (M1) and another group was directly onto rooting medium (M2). Only half MS medium without plant growth regulators was employed as control. The cultures were performed on a culture condition the same to initial culture stage. Number of root, root length, root diameter and plant height were recorded in second experiment. After 8 weeks of culture, plantlets were acclimatized and their survival percent was collected in ex vitro condition. In vitro rooted plantlets were planted in wooden tray containing 2:1 compost and sand mixture and maintained under fully shaded condition at ith relative humidity for ee s follo ed by net house condition at and relative humidity for 4 weeks.\r In experiment 1, 0.15 mg· L-1 TDZ produced 100 % survival percent, 70.58 % normal shoot formation percent and 2.25 number of shoot per explant during initial culture. Both BAP and TDZ concentration treated in initial culture increased number of shoots, shoot length and shoot fresh weight by two times in S1. In S2, numbers of shoots and shoot length continuously increased, but shoot fresh weight declined in comparison to S1.\r In experiment 2, 1.0 mg· L-1 NAA (M1) gave maximum number of roots (17.00), and combination of 1.0 mg· L-1 NAA and 0.5 mg· L-1 IBA (M2) produced the highest number of roots (18.16). While application of auxins resulted in reduced root length and increased root diameter, control treatment provided the longest root length and decreased root diameter. In acclimatization stage, 100 % survival was observed in control treatment, NAA and IBA in M1 and combination of NAA and IBA in M2."}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value_mlt":[{"interim":"BAP"}]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-05-05"}],"displaytype":"preview","filename":"hete aung htut MS thesis.pdf","filesize":[{"value":"1290 Kb"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://meral.edu.mm/record/40/files/hete aung htut MS thesis.pdf"},"version_id":"5aa2241c-9876-43f1-b0bb-4fb7317a4a02"}]},"item_1583103131163":{"attribute_name":"Journal articles","attribute_value_mlt":[{}]},"item_1583103147082":{"attribute_name":"Conference papaers","attribute_value_mlt":[{}]},"item_1583103211336":{"attribute_name":"Books/reports/chapters","attribute_value_mlt":[{}]},"item_1583103233624":{"attribute_name":"Thesis/dissertations","attribute_value_mlt":[{"subitem_supervisor(s)":[]}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Htet Aung Htut"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Thesis"}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2014-02"},"item_1583159847033":{"attribute_name":"Identifier","attribute_value":"https://yauor-yau.archive.knowledgearc.net/handle/123456789/263"},"item_title":"In Vitro Propagation of Red Banana Musa acuminata (AAA) cv. Red Dacca","item_type_id":"21","owner":"1","path":["1582969222431"],"publish_date":"2020-03-05","publish_status":"0","recid":"40","relation_version_is_last":true,"title":["In Vitro Propagation of Red Banana Musa acuminata (AAA) cv. Red Dacca"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2021-12-13T01:02:43.852187+00:00"}