{"created":"2020-07-27T07:23:57.836291+00:00","id":2887,"links":{},"metadata":{"_buckets":{"deposit":"aa08c441-e814-49b4-a7cc-98f64d843061"},"_deposit":{"created_by":16,"id":"2887","owner":"16","owners":[16],"owners_ext":{"displayname":"","email":"ayepyonelwin2016@gmail.com","username":""},"pid":{"revision_id":0,"type":"recid","value":"2887"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/2887","sets":["1582963390870:1582967633684"]},"communities":["ccm","ccp","kyauksetu","ltc","maas","miit","mlmu","mmu","mtlu","mtu","mub","mude","mufl","pathein","scu","suoe","tcu","tgu","tuh","tum","ucsm","ucsmtla","ucsmub","ucspathein","ucstaungoo","ucsy","udmm","udmy","uit","um","um1","um2","umkn","umm","uphy","urj","uvs","uy","yau","ydbu","ytu","yude","yueco","yufl","yuoe"],"control_number":"2887","item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Isolation and Identification of BioactivePhytoconstituents and their Biological Activities of Limoniaacidissima L. (Thee)","subitem_1551255648112":"en"}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"Limonia acidissima L. (Thee) has invaluable effect on skin and enormous range of pharmacological activities. Although the efficacy of this bark extract on skin has been studied, no study has been done the skin whitening effect with the sunscreen lotions from the various extracts of this plant. Present study is designed to get the scientific proof of the sunscreen lotions from the herbal extract. Sample collection and identification, preliminarily phytochemical investigation and elemental analysis by AAS method have been carried out. Some biological activities such as antimicrobial activity, acute toxicity and antioxidant activity of bark of L. acidissima (Thee) have been studied. Nutritional values such as moisture content, ash content, protein content, fibre content, fat content, carbohydrate content and energy value of L. acidissima (Thee) were determined. The antimicrobial activity of PE, CHCl3), MeOH, CH3COCH3, EtOAc, EtOH and watery extracts from bark of L. acidissima and MeOH, EtOAc and EtOH extracts from fruit pulp have been preliminarily screened on six microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacilus pulmalis, Candida albicans and Escherichia coli. Acute toxicity of Thee bark was also studied by Litchfield and Wilcoxon method using mice models. From acute toxicity, it has been found that the 95 % ethanol extract of bark was free from toxic effect. Antioxidant activity test assessed by DPPH radical scavenging activity assay revealed ethanol extract of Thee bark possesses more radical scavenging activity than other extracts (PE and EtOAc) when compared to standard antioxidant, ascorbic acid (lCso= 28.4 ug / mL). Three isolated compounds were isolated from ethyl acetate extract of bark of\nL. acidissima (Thee) by thin layer and silica gel column chromatographic methods. The isolated compound I (xanthotoxin, 0.025% yeild), compound II (isopimpinellin, 0.025%) and compound III (mannesin, 0.005%) were identified by physicochemical determination and modern spectroscopic techniques such as UV, FT IR, 1H NMR, 13C NMR, HSQC, HMBC and HR ESI Mass spectrometry as well as by comparing with the reported data. In addition, the isolated compound IV (auraptene) was also isolated from dichloromethane extract of Thee bark and purified by vacuum liquid column chromatography, flash column chromatography and high performance liquid chromatography. The isolated compound IV was identified by modem spectroscopic techniques such as 1H NMR, 13C NMR, HMBC, ESI mass spectrometry as well as comparing with the reported data. The ethanol extract of bark and fruit pulp of L. acidissima (Thee) were formulated as sunscreen lotions. The skin irritation test was performed by using albino rats. The efficacy of sunscreen lotions was determined by UV visible spectrophotometer. Moreover, the skin whitening effect of sunscreen lotions (thee bark and fruit pulp) was studied by adopting the method reported by Yusutami et al., 2004 with some modification. Moreover, the effectiveness of the sunscreen lotions was evaluated using SPF. From the result, sunscreen lotions were proved to be nonirritant, possess SPF for normal skin."}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value_mlt":[{"interim":"Limonia acidissima"},{"interim":"xanthotoxin"},{"interim":"isopimpinellin"},{"interim":"marmesin"},{"interim":"auraptene"},{"interim":"antimicrobial"},{"interim":"antitumor"},{"interim":"antioxidant"},{"interim":"irritation"},{"interim":"sunscreen lotion"},{"interim":"SPF"}]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-07-27"}],"displaytype":"preview","filename":"Khin Chan Thar (TS006319)  (1-167).pdf","filesize":[{"value":"44.4 Mb"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://meral.edu.mm/record/2887/files/Khin Chan Thar (TS006319)  (1-167).pdf"},"version_id":"a34bda93-6c03-442c-a2e9-680665913c72"}]},"item_1583103233624":{"attribute_name":"Thesis/dissertations","attribute_value_mlt":[{"subitem_awarding_university":"University of Yangon, Department of Chemistry"}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Khin Chan Thar"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Dissertation "}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2016-05-01"},"item_title":"Isolation and Identification of BioactivePhytoconstituents and their Biological Activities of Limoniaacidissima L. (Thee)","item_type_id":"21","owner":"7","path":["1582967633684"],"publish_date":"2020-07-27","publish_status":"0","recid":"2887","relation_version_is_last":true,"title":["Isolation and Identification of BioactivePhytoconstituents and their Biological Activities of Limoniaacidissima L. (Thee)"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2021-12-13T04:27:39.628782+00:00"}