{"created":"2020-03-08T05:05:09.062258+00:00","id":152,"links":{},"metadata":{"_buckets":{"deposit":"66c4577c-e3f8-42b1-9e45-8883db577f29"},"_deposit":{"id":"152","owners":[],"pid":{"revision_id":0,"type":"recid","value":"152"},"status":"published"},"_oai":{"id":"oai:meral.edu.mm:recid/152","sets":["1582963567848:1582969177975"]},"communities":["yau"],"control_number":"152","item_1583103067471":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Characterization of Indigenous Green Gram Rhizobium Isolates and Survival on Different Carriers","subitem_1551255648112":"en"}]},"item_1583103085720":{"attribute_name":"Description","attribute_value_mlt":[{"interim":"To evaluate the nodulation efficiency of indigenous rhizobium isolates, to examine morphology and biochemical properties of indigenous rhizobium isolates and to determine the survival of the most effective-indigenous rhizobium isolate on different carriers, the experiments were conducted at Department of Plant Pathology, Yezin Agricultural University from June 2018 to February 2019. A total of 45 soil samples were collected from green gram cultivated fields in Myaing, Pakokku and Yesagyo Townships of Magway Region. Nodulation and plant growth of 'Yezin-11´ green gram variety inoculated with 45 rhizobium isolates were evaluated by using sterile sand culture pots in a completely randomized design with five replications. The results indicated that all indigenous rhizobium isolates were significantly different in their effectiveness in terms of nodule dry weight and shoot dry weight. All indigenous rhizobium isolates were highly effective (> 80%) based on their symbiotic effectiveness (SE%). Based on nodule dry weight and shoot dry weight, 14 rhizobium isolates; M-1, M-9, M-10, M-11, M-12, P-8, P-9, P-12, P-13,  P-15, Y-1, Y-6, Y-9 and Y-15 were selected and their nitrogen (N) uptakes, morphology and biochemical properties were tested. The N uptakes (mg plant-1) were highly significant among rhizobium inoculated plants. All tested isolates showed significant differences in growth rate, colony size, colour and opacity on yeast extract mannitol agar medium. Seven isolates: M-10, M-12, P-8, P-12, P-13, P-15 and Y-6 were fast-growing Rhizobium spp. while the rest: M-1, M-9, M-11, P-9, Y-1, Y-9 and Y-15 were slow-growng Bradyrhizobium spp. All 14 rhizobium isolates were gram-negative and rod-shaped. They were able to grow in 2% sodium chloride concentration and on glucose peptone agar. Positive results were found in catalase and urease tests whereas negative reactions for methyl red except P-15. Starch hydrolysis test was negative except for M-1, M-12, P-8 and P-9. All of the fast-growing Rhizobium spp. and 57.14% of the slow-growing Bradyrhizobium spp. were able to fix more nitrogen than N (+) control. Survival of M-11 isolate which had the highest symbiotic effectiveness was assessed in different carriers: charcoal, clay soil, fly ash, Australia peat soil, Myanmar peat soil, press mud and saw dust at 15-day interval until 6 months at room temperature. Charcoal, clay soil, Australia peat soil and Myanmar peat soil retained the acceptable rhizobial population, 106 cfu g-1 at the end of the storage period. Therefore, charcoal and clay soil could be considered as the alternative carriers for the future rhizobium inoculant production."}]},"item_1583103108160":{"attribute_name":"Keywords","attribute_value_mlt":[{"interim":"Characterization of Indigenous Green Gram Rhizobium Isolates and Survival on Different Carriers"}]},"item_1583103120197":{"attribute_name":"Files","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-05-05"}],"displaytype":"preview","filename":"Kyi Kyi Thein_Thesis (PTY-95).pdf","filesize":[{"value":"2608 Kb"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://meral.edu.mm/record/152/files/Kyi Kyi Thein_Thesis (PTY-95).pdf"},"version_id":"4baa0800-048f-48e6-96fb-0dd3c02d0247"}]},"item_1583103131163":{"attribute_name":"Journal articles","attribute_value_mlt":[{"subitem_journal_title":"Yezin Agricultural University"}]},"item_1583103147082":{"attribute_name":"Conference papaers","attribute_value_mlt":[{}]},"item_1583103211336":{"attribute_name":"Books/reports/chapters","attribute_value_mlt":[{}]},"item_1583103233624":{"attribute_name":"Thesis/dissertations","attribute_value_mlt":[{"subitem_supervisor(s)":[]}]},"item_1583105942107":{"attribute_name":"Authors","attribute_value_mlt":[{"subitem_authors":[{"subitem_authors_fullname":"Thein, Kyi Kyi"}]}]},"item_1583108359239":{"attribute_name":"Upload type","attribute_value_mlt":[{"interim":"Publication"}]},"item_1583108428133":{"attribute_name":"Publication type","attribute_value_mlt":[{"interim":"Thesis"}]},"item_1583159729339":{"attribute_name":"Publication date","attribute_value":"2019-10"},"item_1583159847033":{"attribute_name":"Identifier","attribute_value":"https://yauor-yau.archive.knowledgearc.net/handle/123456789/196"},"item_title":"Characterization of Indigenous Green Gram Rhizobium Isolates and Survival on Different Carriers","item_type_id":"21","owner":"1","path":["1582969177975"],"publish_date":"2020-03-05","publish_status":"0","recid":"152","relation_version_is_last":true,"title":["Characterization of Indigenous Green Gram Rhizobium Isolates and Survival on Different Carriers"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-03-24T23:12:06.134789+00:00"}