2024-03-29T05:59:43Z
https://meral.edu.mm/oai
oai:meral.edu.mm:recid/134
2021-12-13T00:46:19Z
1582963567848:1582969222431
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In Vitro Clonal Propagation of Phalaenopsis through Flower Stalk Node and Young Leaf
May Kaung Hsu Thwe
Phalaenopsis orchid, an elegant tropical orchid, has high price in flower
markets around the world as well as Myanmar. It is difficult to propagate vegetatively
in nature due to its growth habit. Nowadays, tissue culture technique has been widely
used for most rare species and species that are difficult to propagate Therefore, tissue
culture became an important method for propagation of Phalaenopsis. Tissue culture
techniques, explant types, plant growth regulators and culture systems are mainly
attributed to success. Two experiments were carried out by using different flower
stalk nodes at different positions and in vitro young leaves. The objective of the
research is to investigate the effect of plant growth regulator, culture systems and
media in in vitro regeneration.
In flower stalk node culture, the effects of 1st, 2nd, 3rd, 4th, 5th and 6th nodal
positions counted from base to top were tested for shoot regeneration. In leaf culture,
two culture systems (solid and cotton support medium) and different concentrations
and combinations of benzyladenine (BA) 2 and 4 mg.L-1, thidiazuron (TDZ) 1 and
2 mg.L-1, with and without naphthaleneacetic acid (NAA) 0.5 mg.L-1 were tested for
protocorm like bodies (PLBs) induction from leaf explant. In PLB proliferation stage,
three culture systems (solid, liquid and cotton support liquid culture medium) were
tested. Vecin and Went (VW) and Murashige & Skoog (MS) basal medium with and
without 0.1 mg.L-1 BA and 1 mg.L-1 NAA were tested in rooting stage of shoot
developed from leaf culture.
Among the flower stalk node positions, better responses were observed from
2nd nodal position in shoot multiplication. 3rd nodal position gave best results in root
regeneration and hardening stages. Using flower stalk node culture for mass
propagation, 2nd, 3rd and 4th middle nodal positions should be used as the explants for
production of Phalaenopsis amabilis orchid. In leaf culture, maximum number of
PLBs per explant (7.1) was observed from medium supplemented with 2 mg.L-1 BA.
Culture systems used in initial culture were not statistically difference among each
other. But solid culture showed better results than the cotton culture. The interaction
effect between PGR and culture systems was observed in survival % and number of
PLBs per explants. The results indicate the used of PGRs vary according to the
culture systems. Therefore, BA should be used in cotton culture and TDZ in solid
culture. Cotton culture gave the best result in PLBs proliferation on leaf explants. VW
medium showed better results than MS medium in root formation of PLBs.
2017-08
http://hdl.handle.net/20.500.12678/0000000134
https://meral.edu.mm/records/134