2026-06-24T21:48:49Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/891 2021-12-13T03:57:42Z 1582963739756:1582967007954 user-um

Snake Venom Inhibition Activity of Rosmarinic acid from Argusia argentea A methanolic extract of Argusia argentea significantly inhibited hemorrhage induced by crude venom of Protobothrops flavoviridis (Habu). The extract was then separated according to antivenom activity by using silica gel column chromatography and HPLC equipped with an octadecylsilanized silica gel (ODS) column to afford rosmarinic acid (RA) (1) as an active principle. Anti-hemorrhagic activity was assayed by using several kinds of snake venom. RA (1) significally inhibited the hemorrhagic effect of crude venoms of P. flavoviridis, Crotalus atrox, Gloydius blomhoffii, Bitis arietans as well as purified snake venom metalloproteinases, HT-b (Crotalus atrox), bilitoxin-2 (Agkistrodon bilineatus), HT-1 (Bitis arietans) and Ac1-proteinase (Deinagkistrodon acutus). Inhibition against fibrinogen hydrolytic and collagen hydrolytic activities of P. flavoviridis venom were examined by SDS-PAGE. A histopathological study was done by microscopy after administration of venom in the presence or absence of RA (1). RA (1) was found to markedly neutralize venom-induced hemorrhage, fibrinogenolysis, cytotoxicity, lethality, edema and digestion of type IV collagen activity. Moreover, RA (1) inhibited both hemorrhage and neutrophil infiltrations caused by P. flavoviridis venom in pathology sections. These findings indicate that RA (1) can be expected to provide therapeutic benefits in neutralization of snake venom accompanied by heat stability. 2011 http://hdl.handle.net/20.500.12678/0000000891 https://meral.edu.mm/records/891