2026-04-16T01:57:17Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/55 2021-12-13T01:40:31Z 1582963567848:1582969222431 user-yau

In Vitro Regeneration of Selected Rice (Oryza sativa L.) Genotypes through Anther Culture Khin Soe Win Rice is one of the most important cereal crops in the world. Anther culture, one of double haploid techniques, is simplest and most efficient technique for rice breeding. The present study was carried out to evaluate callus induction ability of selected rice genotypes on media supplemented with two different carbon sources and to investigate suitable BAP concentrations on plant regeneration of anther derived callus. The selected 19 rice genotypes (14 indica and 5 tropical japonica) were used in the experiment. Anthers of each genotype were cultured on N6 medium supplemented with two types of carbon source (4% maltose or 4% sucrose), 2mg.L-1 2,4- Dichlorophenoxyacetic acid (2,4-D) and 0.5 mg.L1 kinetin for callus induction. Anther derived callus was transferred to Murashige and Skoog (MS) medium supplemented with (0, 0.5, 1) mg.L-1 6-Benzylaminopurine (BAP), 1mg.L-11- Naphthaleneacetic acid (NAA) and 1mg.L-1 l kinetin for regeneration. Seventeen out of 19 rice genotypes produced callus on media supplemented with maltose while only 13 genotypes produced callus on media supplemented with sucrose due to genotype dependency. Tropical japonica was more responsive to induce callus from anther than indica among the tested genotypes. Callus induction varied from 0.44% to 19.22%. Tropical japonica (Paw San Taung Pyan Hmwe) has highest callus induction (19.22%) than the other tested genotypes. Yebaw Sein has highest callus induction (6.78%) among the tested indica rice genotypes. Plant regeneration from callus varied from 0% to 25.15%. In tropical japonica genotypes, Bay Kyar Taung Pyan was recorded maximum green plants regeneration (25.15%), while Hnan Kar was (13.33%) among indica genotypes. In this experiment, both MS media supplemented with 0.5mg.L-1 BAP and 1mg.L-1 BAP showed maximum green plants regeneration although maximum green spots formation occurred only on 1mg.L-1 BAP. Maximum green plant was found in Bay Kyar Taung Pyan in tropical japonica and Lat Yone Kyi in indica rice. 2017-07 http://hdl.handle.net/20.500.12678/0000000055 https://meral.edu.mm/records/55