2026-04-15T06:52:13Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/341 2021-12-13T01:29:16Z 1582963788001:1582966577571 user-ydbu

Expression and Application of Peptide Materials Containg Functionalizing Sequence as a Metallothionein Cho Lay Sint The present research focus on the peptides that are rich in cysteine or histidine amino acids, which are potential ligands for metal ions. These peptide sequence were designed from the nine kinds of metal binding fragments of xenopas laevies transcription factor III A zinc finger protein. The protein material was prepared by the rolling circle amplification (RCA) which was a useful and simple technique to amplify oligonucleotide for the biomaterials containing tandem repetitive sequence. The purification and characterization of protein were expressed in E.coli. This study targeted on the functionalized protein having the repetitive sequence of ( YICSFADCGAAYNKNWKLQAHLCKH )n as the RCA generated protein materials. The procedure for the purification of histidine tagged proteins has been developed using metal chelating affinity chromatography. This purification method can be used for proteins containing histidine tag and yielding high amounts of purified protein. 2007 http://hdl.handle.net/20.500.12678/0000000341 https://meral.edu.mm/records/341