2026-04-19T09:04:01Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/3128 2021-12-13T05:47:47Z 1582963366982:1597649530495 user-um1

Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D 3 or 9- cis -retinoic acid in undifferentiated THP-1 cells Kyu Kyu Maung Akira Miyazaki Hisayuki Nomiyama Catherine C. Y. Chang Ta-Yuan Chang Seikoh Horiuchi <p>Abstract We have previously shown that acyl-coenzyme A:&nbsp;cholesterol acyltransferase-1 (ACAT-1) protein content increases<br> significantly during the human monocyte-macrophage&nbsp;differentiation process. To gain further insight, we&nbsp;used undifferentiated human monocytic THP-1 cells as a&nbsp;model system with which to examine whether ACAT-1&nbsp;mRNA and protein content can be increased by treating&nbsp;cells with 1,25-dihydroxyvitamin D&nbsp;3&nbsp;[1,25-(OH)&nbsp;2D3] or with&nbsp;9-cis&nbsp;-retinoic acid (9-cis-RA), two agents known to upregulate<br> the expression of various genes during the monocyte-macrophage&nbsp;differentiation process. Immunoblot analysis with&nbsp;anti-human ACAT-1 antibodies revealed that ACAT-1 protein&nbsp;was increased by 2.6-fold, using 1,25-(OH)&nbsp;2D3&nbsp;at a physiological&nbsp;concentration (100 pM). ACAT-1 protein was also&nbsp;increased when using 9-cis-RA, but only at relatively high&nbsp;concentrations (0.1&ndash;1M).</p> 2019-05-21 http://hdl.handle.net/20.500.12678/0000003128 https://meral.edu.mm/records/3128