2026-05-01T15:59:21Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/226 2021-12-13T01:25:04Z 1582963567848:1582969222431 user-yau

Androgenesis of Selected Indica Rice (Oryza sativa L.) Genotypes and F1 Hybrids Hsu Yi Mon This experiment was carried out to evaluate the callusing ability and green plant formation of selected indica rice genotypes and F1 progenies after crossing selected genotypes. Shwe Man-1, Sin Akari-3, Sin Thwe Latt, Thee Htat Yin and Yar-8 genotypes were selected as parental lines based on callusing ability of anther culture. Five crosses (Shwe Man-1 x Thee Htat Yin, Thee Htat Yin x Sin Thwe Latt, Sin Thwe Latt x Sin Akari-3, Yar-8 x Thee Htat Yin and Sin Thwe Latt x Yar-8) were obtained by crossing the selected indica rice genotypes. Anthers of selected indica rice genotypes and F1 hybrids were cultured on N6 medium with supplemented 2 mg·L-1 2,4-D and 0.5 mg·L-1 kinetin for callus induction. Calli were transferred onto MS medium with 1 mg·L-1 IAA, 1 mg·L-1 NAA, 1 mg·L-1 BAP and 2 mg·L-1 kinetin. All tested genotypes showed callus formation except one cross (Sin Thwe Latt x Sin Akari-3) that showed no response on anther culture. Callus induction percentage of responsive genotypes varied from 0.1-2.6%. Green plant formation from the regenerated calli varied from 6.3-37.0%. Among parents, Yar-8 and Shwe Man-1 gave the highest callus induction and green plant formation. Cross involving Yar-8 as female parent, Yar-8 x Thee Htat Yin increased callusing and green plant formation ability. These three genotypes (Yar-8, Shwe Man-1 and cross of Yar-8 x Thee Htat Yin) produced green plants. The anther-derived plants of these three genotypes were haploid and double haploid. According to the results, Yar-8 genotype has the potential for double haploid production through anther culture in rice breeding program. 2017-06 http://hdl.handle.net/20.500.12678/0000000226 https://meral.edu.mm/records/226