2026-06-07T22:29:27Z https://meral.edu.mm/oai

oai:meral.edu.mm:recid/00008411 2022-11-30T06:41:20Z 1582963788001:1582966466300

"Structural Identification and Evaluation of Some Biological Activities of Cyclo (D-Pro-L-Val) from thePseudomonas aeruginosa Schroeter" Su Swe Su Suu Suu Win Chaw Theingi Khin "This research deals with the studies of identification and evaluation of some biological activities of bioactive secondary metabolite compound of Pseudomonas aeruginosa isolated from the clinical soil sample. The soil sample was collected from Insein General Hospital, Yangon Region. The isolated P. aeruginosa strain was characterized by microscopic examination, biochemical tests and confirmed by 16S rRNA sequencing technique. The chloroform extract was prepared from the P. aeruginosa cultured in a large scale of nutrient broth medium.Diketopiperazines, such as, cyclo-(D-Pro-L-Val) was isolatedfrom the silica gel chromatographic separation on the chloroform extract. The isolated secondary metabolite was structurally identified by using modern NMR spectroscopic techniques such as 1H NMR(Proton Nuclear Magnetic Resonance) , 13C NMR (Carbon-13 Nuclear Magnetic Resonance spectroscopy), COSY (¹H-¹H Correlation Spectroscopy), HSQC (Heteronuclear Single Quantum Coherence), ROESY (Rotating frame Overhause Effect Spectroscopy), HMBC spectroscopies (Heteronuclear Multiple Bond Correlation), and MS (mass spectrometry). The isolated compound, cyclo(D-Pro-L-Val) (IC50 = 0.93 μg /mL) was found to exhibit good in vitro cytotoxic activity against human lung cancercell A-549. In addition, cyclo(D-Pro-LVal) (IC50 = 64.12 μM) has mild inhibited in anti-inflammatoryactivity." 2022-08-12 https://meral.edu.mm/records/8411